Issue |
Parasite
Volume 31, 2024
|
|
---|---|---|
Article Number | 51 | |
Number of page(s) | 9 | |
DOI | https://doi.org/10.1051/parasite/2024051 | |
Published online | 29 August 2024 |
Supplementary material
Figure S1: Sequence analyses of CpLTL. (A) Domain organization of CpLTL protein using SMART. SP: signal peptide, TM: transmembrane domain. (B) Amino acid sequences of apicomplexan parasite LTLs were aligned with CpLTL using the Clustal W algorithm. The metal-binding sites were marked with a red arrow, and the carbohydrate-binding sites were marked with a black arrow. Amino acid identities with orthologs from other Cryptosporidium species and apicomplexan parasites. |
Figure S2: Expression of recombinant CpLTL in Escherichia coli and identification of native CpLTL in C. parvum sporozoites. (A) PCR amplification of the CpLTL of C. parvum. Lane M: 2000 bp DNA ladder maker. Lane 1: CpLTL product. (B) Purity of recombinant CpLTL assessed by SDS-PAGE analysis. Lane M: molecular weight markers, Lane 1: CpLTL purified using Glutathione Sepharose affinity chromatography. (C) Expression of recombinant CpADF in E. coli determined via western blotting analysis using an anti-GST tag. Lane M: protein size makers. Lane 1: recombinant CpADF protein. (D) Western blotting analysis of native CpLTL in C. parvum sporozoites using pre-immune serum (left panel) and polyclonal antibodies (right panel). Lane M: protein size makers. Lane 1 and lane 2: native protein from sporozoites. |
Figure S3: Identification of host proteins interacting with CpLTL by GST-pull down combined with LC-MS/MS. (A) Silver-stained one-dimensional SDS-PAGE analysis of GST-CpLTL. Line M: marker. Line 1: GST-CpLTL. Line 2: GST control. Line 3: PBS control. (B) The Venn diagram of the differentiated proteins was obtained by LC-MS/MS identification. |
Table S1: List of proteins identified by LC-MS/MS analysis from the bands recognized by CpLTL in GST-pull down assay. Access here
© X. Zhang et al., published by EDP Sciences, 2024
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.