Figure S2:
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Expression of recombinant CpLTL in Escherichia coli and identification of native CpLTL in C. parvum sporozoites. (A) PCR amplification of the CpLTL of C. parvum. Lane M: 2000 bp DNA ladder maker. Lane 1: CpLTL product. (B) Purity of recombinant CpLTL assessed by SDS-PAGE analysis. Lane M: molecular weight markers, Lane 1: CpLTL purified using Glutathione Sepharose affinity chromatography. (C) Expression of recombinant CpADF in E. coli determined via western blotting analysis using an anti-GST tag. Lane M: protein size makers. Lane 1: recombinant CpADF protein. (D) Western blotting analysis of native CpLTL in C. parvum sporozoites using pre-immune serum (left panel) and polyclonal antibodies (right panel). Lane M: protein size makers. Lane 1 and lane 2: native protein from sporozoites.
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