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Table 3

Percentage identity of rRNA gene subunits/spacers and mitochondrial genes within and between Dibothriocephalus dendriticus and Dibothriocephalus ditremus.

DNA region Length D. dendriticus (% identity) D. ditremus (% identity) D. dendriticus/D. ditremus (% identity)
rRNA gene subunits and spacers
 ssrDNA Partial; 2,024 bpa 100 99.9 99.8–100
 lsrDNA Partial; 1,413 bpa 99.9–100 99.8–100 99.7–99.9
ITS1 rDNA Partial; 505 bpa 99.0–100 99.2–100 99.4–100
ITS2 rDNA Complete; 464–480 bpa, b 98.3–100 96.3–100 96.7–99.6
Mitochondrial genes
cox1 mtDNA Complete; 1,566 bpc 96.2–100 96.0–96.9 89.7–91.3
 cob mtDNA Complete; 1,107 bpc 97.3–100 97.0–98.9 89.3–90.9

ssrDNA, small subunit of rRNA gene; lsrDNA, large subunit of rRNA gene; ITS1, internal transcribed spacer 1; ITS2, internal transcribed spacer 2; cox1, cytochrome c oxidase subunit 1; cob, cytochrome b.

a

Supplementary Table 1 indicates sequences (see “+” mark in “% identity” column) that were trimmed to the uniform length and used for calculation of the percentage identity;

b

The length of ITS2 spacer varied from 464 bp to 480 bp due to different number of repetitive motifs and insertions/deletions;

c

Supplementary Table 2 indicates complete cox1 and cob sequences (see “+” mark in “% identity” column) which were used for calculation of the percentage identity.

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