| Issue |
Parasite
Volume 32, 2025
|
|
|---|---|---|
| Article Number | 41 | |
| Number of page(s) | 13 | |
| DOI | https://doi.org/10.1051/parasite/2025033 | |
| Published online | 04 July 2025 | |
Supplementary material
Supplementary Table 1: Summary data on the sequences of the rRNA gene subunits and spacers of Dibothriocephalus dendriticus and Dibothriocephalus ditremus retrieved from GenBank. Access here
Supplementary Table 2: Summary data on the sequences of the mitochondrial DNA genes of Dibothriocephalus dendriticus and Dibothriocephalus ditremus retrieved from GenBank. Access here
Supplementary Table 3: Mutation sites (species-specific mutations are in red) and design of the species-specific primers (in colored boxes) in the cox1 gene of Dibothriocephalus dendriticus and Dibothriocephalus ditremus. Access here
Supplementary Table 4: Mutation sites (species-specific mutations are in red) and design of the species-specific primers (in colored boxes) in the cob gene of Dibothriocephalus dendriticus and Dibothriocephalus ditremus. Access here
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Supplementary Figure 1: PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using (A) D. dendriticus-specific (Dde_cox1_F; Dde_cox1_R) and (B) D. ditremus-specific (Ddi_cox1_F; Ddi_cox1_R) cox1 primers designed in the current study. Lane M: 100 bp ladder. |
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Supplementary Figure 2: PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using the first set of (A) D. dendriticus-specific (Dde_cob_F1; Dde_cob_R1) and (B) D. ditremus-specific (Ddi_cob_F1; Ddi_cob_R1) cob primers designed in the current study. Lane M: 100 bp ladder. |
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Supplementary Figure 3: PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using the second set of (A) D. dendriticus-specific (Dde_cob_F2; Dde_cob_R2) and (B) D. ditremus-specific (Ddi_cob_F2; Ddi_cob_R2) cob primers designed in the current study. Lane M: 100 bp ladder. |
 |
Supplementary Figure 4: PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using (A) primers amplifying the microsatellite locus Dd_38 and (B) primers amplifying the microsatellite locus Dd_78 designed by Bazsalovicsová et al. (2020) for D. dendriticus. Lane M: 100 bp ladder. |
© L. Králová-Hromadová et al., published by EDP Sciences, 2025
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