Open Access
Issue
Parasite
Volume 32, 2025
Article Number 41
Number of page(s) 13
DOI https://doi.org/10.1051/parasite/2025033
Published online 04 July 2025

Supplementary material

Supplementary Table 1: Summary data on the sequences of the rRNA gene subunits and spacers of Dibothriocephalus dendriticus and Dibothriocephalus ditremus retrieved from GenBank. Access here

Supplementary Table 2: Summary data on the sequences of the mitochondrial DNA genes of Dibothriocephalus dendriticus and Dibothriocephalus ditremus retrieved from GenBank. Access here

Supplementary Table 3: Mutation sites (species-specific mutations are in red) and design of the species-specific primers (in colored boxes) in the cox1 gene of Dibothriocephalus dendriticus and Dibothriocephalus ditremus. Access here

Supplementary Table 4: Mutation sites (species-specific mutations are in red) and design of the species-specific primers (in colored boxes) in the cob gene of Dibothriocephalus dendriticus and Dibothriocephalus ditremus. Access here

thumbnail Supplementary Figure 1:

PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using (A) D. dendriticus-specific (Dde_cox1_F; Dde_cox1_R) and (B) D. ditremus-specific (Ddi_cox1_F; Ddi_cox1_R) cox1 primers designed in the current study. Lane M: 100 bp ladder.

thumbnail Supplementary Figure 2:

PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using the first set of (A) D. dendriticus-specific (Dde_cob_F1; Dde_cob_R1) and (B) D. ditremus-specific (Ddi_cob_F1; Ddi_cob_R1) cob primers designed in the current study. Lane M: 100 bp ladder.

thumbnail Supplementary Figure 3:

PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using the second set of (A) D. dendriticus-specific (Dde_cob_F2; Dde_cob_R2) and (B) D. ditremus-specific (Ddi_cob_F2; Ddi_cob_R2) cob primers designed in the current study. Lane M: 100 bp ladder.

thumbnail Supplementary Figure 4:

PCR amplification of genomic DNA of Dibothriocephalus dendriticus (Dde) and Dibothriocephalus ditremus (Ddi) from Takvatn lake, Norway (NO-TA), Kalandsvatn lake, Norway (NO-KA), Hafravatn lake, Iceland (IS-HA) and Thingvallavatn lake, Iceland (IS-TH) using (A) primers amplifying the microsatellite locus Dd_38 and (B) primers amplifying the microsatellite locus Dd_78 designed by Bazsalovicsová et al. (2020) for D. dendriticus. Lane M: 100 bp ladder.


© L. Králová-Hromadová et al., published by EDP Sciences, 2025

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