| Issue |
Parasite
Volume 32, 2025
|
|
|---|---|---|
| Article Number | 16 | |
| Number of page(s) | 15 | |
| DOI | https://doi.org/10.1051/parasite/2025003 | |
| Published online | 26 February 2025 | |
Supplementary material
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Figure S1. Analysis of cytokine/chemokine levels in serum of ds-RNA treated mice. Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized at a specific time after NaCl or ds-GFP treatment and sera were collected. Levels of cytokines or chemokines in the serum were detected using the Luminex liquid suspension chip assay. ns p > 0.05 by unpaired t-test. n = 4. Error bars represent the SEM. |
 |
Figure S2. RT-qPCR analysis of proinflammatory genes in lung of mice treated with ds-GFP or NaCl. Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized 28 days after NaCl or ds-GFP treatment. The lung was collected and total RNA was extracted by TRIzol reagent. The relative mRNA expression of TGF-β (A), IL-10 (B), IL-33 (C), IL-1β (D), IFN-γ (E), iNOS (F), IL-6 (G), and IL-17A (H) in the spleen was detected by qPCR. ns p > 0.05 by unpaired t-test. n = 4. Error bars represent the SEM. |
 |
Figure S3. RT-qPCR analysis of proinflammatory genes in heart of mice treated with ds-GFP or NaCl. Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized 28 days after NaCl or ds-GFP treatment. The heart was collected and total RNA was extracted by TRIzol reagent. The relative mRNA expression of TGF-β (A), IL-10 (B), IL-33 (C), IL-1β (D), IFN-γ (E), iNOS (F), IL-6 (G), and IL-17A (H) in the spleen was detected by qPCR. ns p > 0.05 by unpaired t-test. n = 4. Error bars represent the SEM. |
 |
Figure S4. RT-qPCR analysis of proinflammatory genes in kidney of mice treated with ds-GFP or NaCl. Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized 28 days after NaCl or ds-GFP treatment. The kidney was collected and total RNA was extracted by TRIzol reagent. The relative mRNA expression of TGF-β (A), IL-10 (B), IL-33 (C), IL-1β (D), IFN-γ (E), iNOS (F), IL-6 (G), and IL-17A (H) in the spleen was detected by qPCR. ns p > 0.05 by unpaired t-test. n = 4. Error bars represent the SEM. |
 |
Figure S5. RT-qPCR analysis of proinflammatory genes in liver of mice treated with ds-GFP or NaCl. Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized 28 days after NaCl or ds-GFP treatment. The liver was collected and total RNA was extracted by TRIzol reagent. The relative mRNA expression of TGF-β (A), IL-10 (B), IL-33 (C), IL-1β (D), IFN-γ (E), iNOS (F), IL-6 (G), and IL-17A (H) in the spleen was detected by qPCR. ns p > 0.05 by unpaired t-test. n = 4. Error bars represent the SEM. |
Supplementary Table 1. All primers for this study.
Supplementary Table 2. GFP_spleen_VS_NaCl_spleen_DEGenes and GFP_spleen_VS_NaCl_spleen_GOterms_enrichment.
Supplementary Table 3. dsGFP.F_VS_NACL.F_DEGenes and dsGFP.M_VS_NACL.M_DEGenes.
Access here© L. Xiu et al., published by EDP Sciences, 2025
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