RT-qPCR analysis of proinflammatory genes in heart of mice treated with ds-GFP or NaCl.

Eight mice were randomly divided into the NaCl group and the ds-GFP group, with each mouse being infected percutaneously with 60 ± 2 cercariae. The mice were euthanized 28 days after NaCl or ds-GFP treatment. The heart was collected and total RNA was extracted by TRIzol reagent. The relative mRNA expression of TGF-β (A), IL-10 (B), IL-33 (C), IL-1β (D), IFN-γ (E), iNOS (F), IL-6 (G), and IL-17A (H) in the spleen was detected by qPCR. ns p > 0.05 by unpaired t-test. n = 4. Error bars represent the SEM.