Supplementary material

Figure S1: Locality map of Yunnan and flow chart of experiment. (A) Sketch map of Yunnan Province, China. Major rivers and mountains are shown roughly by green and grey lines, respectively. (B) Collecting samples on Farm A. (C) Preliminary RT-qPCR test for Culicoides. (D) Further RT-qPCR test for positive samples.

Figure S2: Identification of viral isolates by RT-PCR.

Table S1: Details of collection sites in Lufeng County, Yunnan Province, China. Access here

Table S2: Blood samples and insect collections collected in this study. Access here

Table S3: High quality contigs and PCR fragments acquired in this study. Access here

Table S4: Novel primers and probes designed in this study. Access here

Table S5: Details of the pools of Culicoides used in virus isolation attempts. Access here

Table S6: Species composition and size of pools of Culicoides processed for the presence of virus by RT-qPCR. Access here

Supplemental File 1: Methods and results to confirm BTV in Culicoides. Access here

© Y.-L. Duan et al., published by EDP Sciences, 2024