Supplementary materials

Supplementary Table 1

Farmer registry list used during blood samples collection in the districts of Kasungu and Nkhotakota. (*) indicates the data obtained from the District Agriculture Development Office (DADO) for each district through their District Animal Health and Livestock Development Officer (DAHLDO). (Access here)

Supplementary Table 2

List of primers and their sequences used in this study. (Access here)

Supplementary Table 3

SRA PCR, ITS PCR, and MinION sequencing analysis for characterizations of trypanosome species. No hit* shows the number of reads that could not be identified by BLAST. FASTA $ describes the total number of obtained reads processed into BLAST. “PC+” and “?” refer to positive control and ambiguous results, respectively. Samples which were negative in both the SRA PCR and the ITS1 PCR are not shown here. (Access here)

Supplementary Figure

Workflow for molecular detection of African trypanosomes. The workflow depicts structural steps for detection of African animal and human trypanosomes with serial arrows to the right for cattle samples, and a single downward arrow for human samples. The section on the identification of trypanosome species by sequencing is divided into three steps: library preparation, sequencing and basecalling, and de-multiplexing. Bioinformatic analyses required in the experiments are framed in red borderline. (Access here)

© M. Marsela et al., published by EDP Sciences, 2020