Volume 25, 2018
|Number of page(s)||12|
|Published online||26 November 2018|
Sequence alignment of the three siRNAs with the EgTPx sequence.
Genotypic characterisation of Echinococcus granulosus s.l. isolates using the cytochrome oxidase 1 (cox1) gene. A, Gel electrophoresis of amplified PCR products of protoscoleces used in this study. M: Indicates 5000 bp DNA Marker; Lane S1-3: protoscoleces samples; Lane 4: Negative control; B, Phylogenetic tree with the protoscoleces used in this study (denoted as S1–S3) based on the cox1 gene, with reference sequences for 7 different genotypes from GenBank (G1 – NC00928, G3 – KJ559023, G4 – AF346403, G5 – AB235846, G6 – AB208063, G7 – AB235847a and G8 – AB235848); C, Alignment positions of the amplified mitochondrial cox1 gene sequences in comparison with available published sequences of Echinococcus granulosus s.l. genotypes G1–G8. Dots indicate identity with the G1 (Echinococcus granulosus s.s., common sheep strain) sequence chosen as the reference.
rEgTPx expression and Dot-blot analysis of the purified mouse anti-rEgTPx serum. A, Agarose gel electrophoresis image of the PCR-amplified EgTPx gene. Lane1: DNA Marker/DL2 000 (Takara, Otsu, Japan); Lane2: EgTPx was successfully amplified; Lane3: Without template as native control. B, SDS-PAGE analysis of rEgTPx. M: Protein markers; Lane 1: Purified rEgTPx protein. C, Dot-blot analysis of the EgTPx antiserum before purification. D, Dot-blot analysis of the EgTPx antiserum after purification; lanes 1, 2 and 3 show the one-third serial dilutions of purified rEgTPx (upper) and the lysates of bacterial cells transformed with the pET-28a expression vector expressing the His tag (lower).
Additional file 1: Video file 1. Fluorescence of Cy3-labelled siRNA in protoscoleces.
© H. Wang et al., published by EDP Sciences, 2018
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