Open Access
Research Article
Issue
Parasite
Volume 25, 2018
Article Number 57
Number of page(s) 12
DOI https://doi.org/10.1051/parasite/2018055
Published online 26 November 2018

Supplementary Materials

thumbnail Figure S1.

Sequence alignment of the three siRNAs with the EgTPx sequence.

thumbnail Figure S2.

Genotypic characterisation of Echinococcus granulosus s.l. isolates using the cytochrome oxidase 1 (cox1) gene. A, Gel electrophoresis of amplified PCR products of protoscoleces used in this study. M: Indicates 5000 bp DNA Marker; Lane S1-3: protoscoleces samples; Lane 4: Negative control; B, Phylogenetic tree with the protoscoleces used in this study (denoted as S1–S3) based on the cox1 gene, with reference sequences for 7 different genotypes from GenBank (G1 – NC00928, G3 – KJ559023, G4 – AF346403, G5 – AB235846, G6 – AB208063, G7 – AB235847a and G8 – AB235848); C, Alignment positions of the amplified mitochondrial cox1 gene sequences in comparison with available published sequences of Echinococcus granulosus s.l. genotypes G1–G8. Dots indicate identity with the G1 (Echinococcus granulosus s.s., common sheep strain) sequence chosen as the reference.

thumbnail Figure S3.

rEgTPx expression and Dot-blot analysis of the purified mouse anti-rEgTPx serum. A, Agarose gel electrophoresis image of the PCR-amplified EgTPx gene. Lane1: DNA Marker/DL2 000 (Takara, Otsu, Japan); Lane2: EgTPx was successfully amplified; Lane3: Without template as native control. B, SDS-PAGE analysis of rEgTPx. M: Protein markers; Lane 1: Purified rEgTPx protein. C, Dot-blot analysis of the EgTPx antiserum before purification. D, Dot-blot analysis of the EgTPx antiserum after purification; lanes 1, 2 and 3 show the one-third serial dilutions of purified rEgTPx (upper) and the lysates of bacterial cells transformed with the pET-28a expression vector expressing the His tag (lower).

Additional file 1: Video file 1. Fluorescence of Cy3-labelled siRNA in protoscoleces.


© H. Wang et al., published by EDP Sciences, 2018

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.