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Genotypic characterisation of Echinococcus granulosus s.l. isolates using the cytochrome oxidase 1 (cox1) gene. A, Gel electrophoresis of amplified PCR products of protoscoleces used in this study. M: Indicates 5000 bp DNA Marker; Lane S1-3: protoscoleces samples; Lane 4: Negative control; B, Phylogenetic tree with the protoscoleces used in this study (denoted as S1–S3) based on the cox1 gene, with reference sequences for 7 different genotypes from GenBank (G1 – NC00928, G3 – KJ559023, G4 – AF346403, G5 – AB235846, G6 – AB208063, G7 – AB235847a and G8 – AB235848); C, Alignment positions of the amplified mitochondrial cox1 gene sequences in comparison with available published sequences of Echinococcus granulosus s.l. genotypes G1–G8. Dots indicate identity with the G1 (Echinococcus granulosus s.s., common sheep strain) sequence chosen as the reference.
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