Figure 2

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Gametocytes of Leucocytozoon macleani, lineages GALLUS17 (A–F) and lineages GALLUS63 (G–H). Leucocytozoon macleani gametocytes develop in two types of host cells, including fusiform (A–D, G–H) and roundish (E–F). Cytoplasm of macrogametocytes (A–B, E, G) usually contains small vacuoles (back arrowhead). Nucleus of macrogametocyte (arrow) contains nucleolus (white arrowhead). Two cytoplasmic processes (CP) present in fusiform host cells. Cytoplasm (C) of roundish host cells is largely occupied by macrogametocytes. Both fusiform and roundish host cells with microgametocytes have features similar to host cells with macrogametocytes. Nuclei of microgametocytes are diffuse and cytoplasm stains paler than in macrogametocytes. In fusiform host cell, macrogametocytes and microgametocytes usually displace the nucleus of host cell (double arrow) toward the periphery (A, C, G–H). In fusiform host cells, macrogametocytes and microgametocytes push nuclei aside, deforming them; the nuclei assume band-like form and lie peripherally like a band extending more than half of the circumference of gametocytes. Methanol-fixed, Giemsa-stained blood films. Scale bar = 10 μm.
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