Table 2
Details on primers used for tests of their specificity for Dibothriocephalus dendriticus and Dibothriocephalus ditremus.
| Gene | F primer name | F primer sequence (5′–3′) | R primer name | R primer sequence (5′–3′) | PCR product size (bp) | Primer designed as specific for | Specificity −/+ | Reference |
|---|---|---|---|---|---|---|---|---|
| cox1 mtDNA | MulDen4 | GTGTTTTTCATTTGATGATGACCAGTC | MulRevCom | ATGATAAGGGAYAGGRGCYCA | ~ 310 | D. dendriticus | − | 1 |
| cox1 mtDNA | Dde_cox1_F | CAGCCTTTGTTGATAAATT | Dde_cox1_R | AGTGAAACACGACTTTTTAGA | ~ 480 | D. dendriticus | − | 2 |
| cox1 mtDNA | Ddi_cox1_F | CGGCTTTTGTTGATAATTT | Ddi_cox1_R | AATGAAACACGACTATTTAAA | ~ 480 | D. ditremus | − | 2 |
| cob mtDNA | Dde_cob_F1 | TGTATGTAGCTGACTCAGAATTGA | Dde_cob_R1 | ATACTTTCAGCTATAGCAGTTAAAA | ~ 290 | D. dendriticus | − | 2 |
| cob mtDNA | Ddi_cob_F1 | TATATGTGGCAGACTCAGAATTAA | Ddi_cob_R1 | ACACTTTCTGCTATTGCAGTTAACA | ~ 290 | D. ditremus | − | 2 |
| cob mtDNA | Dde_cob_F2 | CTATGCTTCGCTCTGTTGAGT | Dde_cob_R2 | GCTCTAAAAGTGCTTGATC | ~ 270 | D. dendriticus | − | 2 |
| cob mtDNA | Ddi_cob_F2 | CCATGCTTCGTTCAGTTGAAT | Ddi_cob_R2 | GCTCTAGAAATATTAGATC | ~ 270 | D. ditremus | − | 2 |
| STR locus Dd_2 | Dd_2_F | CCGACAACAACGCTCTAATCC | Dd_2_R | TGCCATTCAGCAAGGTGGAA | ~ 210 | D. dendriticus | − | 3 |
| STR locus Dd_8 | Dd_8_F | CGTCTATGATCACGCATGTCA | Dd_8_R | CGCTGTAGGATTAGATTCACACG | ~ 250 | D. dendriticus | + | 2 |
| STR locus Dd_17 | Dd_17_F | ACGCTACTGCATAGATCGAGG | Dd_17_R | GCATAACGCGCCAGAAACAA | ~ 240 | D. dendriticus | − | 3 |
| STR locus Dd_23 | Dd_23_F | CACACGCAGAAGTCTAGTTGAC | Dd_23_R | TGTTAGCTTACTTCCGTGGCT | ~ 140 | D. dendriticus | − | 3 |
| STR locus Dd_25 | Dd_25_F | GTTATCCTACGTTGGGCTCCT | Dd_25_R | ATCTGGTTGGGAGAAACAACT | ~ 90 | D. dendriticus | − | 3 |
| STR locus Dd_33 | Dd_33_F | TGTTTGCTCCAGTGCCTCG | Dd_33_R | CTAGCAGCATCAGCAGTGGA | ~ 270 | D. dendriticus | + | 3 |
| STR locus Dd_38 | Dd_38_F | ACTATCACGATGCGCTGACA | Dd_38_R | ATCCTTTGTTCCCTGAGCAG | ~ 250 | D. dendriticus | − | 3 |
| STR locus Dd_43 | Dd_43_F | CAGTCTTTCCGGGTGAAGCT | Dd_43_R | GGTAGCTGCAGTACCGATCA | ~ 210 | D. dendriticus | − | 3 |
| STR locus Dd_47 | Dd_47_F | ACTTCGGATTACTTCATTAACTCAGT | Dd_47_R | TGGTGAACGAAGTCAAACTATGC | ~ 190 | D. dendriticus | − | 3 |
| STR locus Dd_49 | Dd_49_F | ACGTCTGACGACAACTTGGG | Dd_49_R | AAGACCCTGGCCAATACACG | ~ 190 | D. dendriticus | − | 3 |
| STR locus Dd_57 | Dd_57_F | AACATGCGAGTCCCAGGAAG | Dd_57_R | AGCAACGATCTACCGTAAAGCA | ~ 120 | D. dendriticus | − | 3 |
| STR locus Dd_78 | Dd_78_F | GCTTTCGGCCATTTGTGGTC | Dd_78_R | GGGACAATAGGCAGGGTCTG | ~ 270 | D. dendriticus | − | 3 |
| STR locus Dd_84 | Dd_84_F | AGAGGTAATTCATCGAGTTCTCTGA | Dd_84_R | TGACTGTGTACATCCGGTCG | ~ 240 | D. dendriticus | − | 3 |
| STR locus Dd_95 | Dd_95_F | CGTTCACGCTCCAATGATCC | Dd_95_R | AGAGCTTGCTGATGATGGCT | ~ 190 | D. dendriticus | − | 3 |
| STR locus Dd_114 | Dd_114_F | ACTTCAGGTAATCTCCGTGTCC | Dd_114_R | CTAGCGCCAATGGGTAGCTT | ~ 130 | D. dendriticus | − | 3 |
F, forward primer; R, reverse primer; cox1 mtDNA, mitochondrial gene cytochrome c oxidase subunit 1; cob mtDNA, mitochondrial gene cytochrome b; STR, short tandem repeat (microsatellite); +, specificity confirmed; −, specificity not confirmed; 1, Wicht et al. [66]; 2, current study; 3, Bazsalovicsová et al. [10].
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