Open Access
Table 2
The 11 polymorphic microsatellite loci selected for Glossina brevipalpis including repeat motif, primer sequence, number of alleles (NA), allele size range, heterozygosity within subsamples (Hs), total heterozygosity (Ht).
| Locus name | Repeat motif | Primer sequence (5’-3’) | N A | Allele size range (bp)a | H s | H t |
|---|---|---|---|---|---|---|
| Gb5 | AG(21) | F: GTTACTAGTCACTCATAAGCACATG | 13 | 219–247 | 0.851 | 0.899 |
| R: CTCATATATGCTTCTTGTTGGTCC | ||||||
| Gb28 | AG(18) | F: CTACTACCAACTCCAATCAAAGAAG | 12 | 227–281 | 0.799 | 0.867 |
| R: TCTACTCGCTAAGAAGTAAATCCTG | ||||||
| Gb35 | AC(17) | F: TTTCTCCTTAGCCTGCATTAGATAG | 10 | 193–239 | 0.673 | 0.783 |
| R: AAACACACACTAACTGAGAGAGG | ||||||
| Gb48* | AC(16) | F: CTAAACCTCTAGTGAAACAGAAGC | 14 | 229–279 | 0.809 | 0.867 |
| R: CTATAACATAACTCTAACGCTACGG | ||||||
| Gb66 | ATC(16) | F: CAAACCTGTTGCATCTTTGTTTGG | 14 | 202–259 | 0.861 | 0.893 |
| R: CCTCGTCACTACCATCGTC | ||||||
| Gb72 | AC(15) | F: TTGTCACATATATGAGAACGACCTC | 14 | 207–245 | 0.798 | 0.841 |
| R: GAAGTTCACTACAATGTCATCTGC | ||||||
| Gb73 | AC(15) | F: TTTCTGATGTTGGAGAGTGTCTAC | 11 | 228–256 | 0.764 | 0.849 |
| R: CCATTTGATTCACGATAACCAACC | ||||||
| Gb92 | AG(15) | F: GAGAGCGAGAGACAACATTTC | 13 | 278–324 | 0.755 | 0.802 |
| R: TTTCGTTATCTCCCTCTTTCGTTTG | ||||||
| Gb158 | AC(13) | F: CACCTCAGCCTTCATAAATTAACAC | 8 | 187–205 | 0.744 | 0.805 |
| R: GCTAACAAAGAGGAGTATGTAC | ||||||
| Gb165 | AC(13) | F: TTTGGGAGAAACACGTACGAC | 5 | 210–220 | 0.534 | 0.604 |
| R: GTTGTATACTTAAGCAACGCACAC |
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