Nematode eggshells: A new anatomical and terminological framework, with a critical review of relevant literature and suggested guidelines for the interpretation and reporting of eggshell imagery

Open Access

Review

Table 1

Some papers showing that Layer #3 exhibits moderate to very intense reaction to Sudan Black B, suggesting that Layer #3a does contain lipid, contradicting recent suggestions that the “inner eggshell layer” (of the classical trilaminar framework) “is composed [instead] of proteoglycans” [83, 103].

Group and species [authority]	Sudan Black B reaction¹ of the Electron-dense Parietal Coating	References
Tylenchida	+ + +	Perry et al. (1982) [87]
Globodera rostochiensis [122]	+ + +	Perry et al. (1982) [87]
Oxyurida	+ +	Wharton (1979) [113]
Hammerschmidtiella diesingi [44]	+ +	Wharton (1979) [113]
Oxyurida	+ + + + +	Wharton (1979) [115]
Aspiculuris tetraptera [101]	+ + + + +	Wharton (1979) [115]
Trichinelloidea	+ + + +	Wharton and Jenkins (1978) [120]
Trichuris suis [100]	+ + + +	Wharton and Jenkins (1978) [120]

+ +, moderate; + + +, strong; + + + +, intense; + + + + +, very intense reactions.

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