Detection of pathogenic trypanosome species by analysis of SRA PCR and MinION sequencing of ITS1 PCR products.
|Sampling sites||Total number of samples||Number of positive samples
|T. congolense||T. vivax||Trypanozoon
||Any trypanosome species|
|T. b. brucei||T. b. rhodesiense|
|Kasungu||199||5  (2.5%)||0 (0%)||2  (0.4%)||19 (9.5%)||25 (12.6%)|
|Nkhotakota||185||20  (10.8%)||12  (6.5%)||0 (0%)||5 (2.7%)||35 (18.9%)*|
|Lilongwe||62||0 (0%)||0 (0%)||0 (0%)||0 (0%)||0 (0%)|
Numbers in brackets [n] indicate samples with a coinfection involving multiple trypanosomes. Numbers in parentheses represent the prevalence of each trypanosome, or infected cattle per district. Asterisk (*) indicates that the prevalence of in Nkhotakota is significantly higher than in Lilongwe and Kasungu (p < 0.05). Samples with T. theileri are counted as negative in this table.
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