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Research Article

Table 1

Detection of pathogenic trypanosome species by analysis of SRA PCR and MinION sequencing of ITS1 PCR products.

Sampling sites Total number of samples Number of positive samples
T. congolense T. vivax Trypanozoon
Any trypanosome species
T. b. brucei T. b. rhodesiense
Kasungu 199 5 [1] (2.5%) 0 (0%) 2 [1] (0.4%) 19 (9.5%) 25 (12.6%)
Nkhotakota 185 20 [2] (10.8%) 12 [2] (6.5%) 0 (0%) 5 (2.7%) 35 (18.9%)*
Lilongwe 62 0 (0%) 0 (0%) 0 (0%) 0 (0%) 0 (0%)
Total 446 25 12 2 24 60
Prevalence 5.6% 2.7% 0.4% 5.4% 13.4%

Numbers in brackets [n] indicate samples with a coinfection involving multiple trypanosomes. Numbers in parentheses represent the prevalence of each trypanosome, or infected cattle per district. Asterisk (*) indicates that the prevalence of in Nkhotakota is significantly higher than in Lilongwe and Kasungu (p < 0.05). Samples with T. theileri are counted as negative in this table.

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