Table 1
Detection of pathogenic trypanosome species by analysis of SRA PCR and MinION sequencing of ITS1 PCR products.
| Sampling sites | Total number of samples | Number of positive samples |
||||
|---|---|---|---|---|---|---|
| T. congolense | T. vivax | Trypanozoon |
Any trypanosome species | |||
| T. b. brucei | T. b. rhodesiense | |||||
| Kasungu | 199 | 5 [1] (2.5%) | 0 (0%) | 2 [1] (0.4%) | 19 (9.5%) | 25 (12.6%) |
| Nkhotakota | 185 | 20 [2] (10.8%) | 12 [2] (6.5%) | 0 (0%) | 5 (2.7%) | 35 (18.9%)* |
| Lilongwe | 62 | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
| Total | 446 | 25 | 12 | 2 | 24 | 60 |
| Prevalence | 5.6% | 2.7% | 0.4% | 5.4% | 13.4% | |
Numbers in brackets [n] indicate samples with a coinfection involving multiple trypanosomes. Numbers in parentheses represent the prevalence of each trypanosome, or infected cattle per district. Asterisk (*) indicates that the prevalence of in Nkhotakota is significantly higher than in Lilongwe and Kasungu (p < 0.05). Samples with T. theileri are counted as negative in this table.
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