Gel images of the ITS1 PCR results for positive and ambiguous samples (n = 53, including one positive control). The combination of number and letter referred to the sample identity. M and PC represent the abbreviation of 1 kb marker and positive control, respectively. The letter below explains the interpretation of the gel (Gel) and the analysis of MinION sequencing (Seq). C, T. congolense; T, Trypanozoon; V/G, T. vivax or T. godfreyi; S, T. simiae; Th, T. theileri; N, no result; and “?”, ambiguous result.”