Open Access
Issue
Parasite
Volume 32, 2025
Article Number 64
Number of page(s) 13
DOI https://doi.org/10.1051/parasite/2025057
Published online 29 September 2025
  1. BIOVIA. 2020. Discovery Studio Visualizer. Available at https://www.3ds.com/products/biovia/discovery-studio/visualization. [Google Scholar]
  2. Chitnumsub P, Jaruwat A, Talawanich Y, Noytanom K, Liwnaree B, Poen S, Yuthavong Y. 2020. The structure of Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase reveals the basis of sulfa resistance. FEBS Journal, 287(15), 3273–3297. [Google Scholar]
  3. Chusacultanachai S, Thiensathit P, Tarnchompoo B, Sirawaraporn W, Yuthavong Y. 2002. Novel antifolate resistant mutations of Plasmodium falciparum dihydrofolate reductase selected in Escherichia coli. Molecular and Biochemical Parasitology, 120(1), 61–72. [Google Scholar]
  4. Coatney GR, Collins WE, Warren M, Contacos PG. 1971. The primate malarias. US National Institute of Allergy and Infectious Diseases. Rockville. Bethesda: US Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health. [Google Scholar]
  5. Contreras CE, Cortese JF, Caraballo A, Plowe CV. 2002. Genetics of drug-resistant Plasmodium falciparum malaria in the Venezuelan state of Bolivar, American Journal of Tropical Medicine and Hygiene, 67(4), 400–405. [Google Scholar]
  6. Genheden S, Ryde U. 2015. The MM/PBSA and MM/GBSA methods to estimate ligand-binding affinities. Expert Opinion on Drug Discovery, 10(5), 449–461. [Google Scholar]
  7. Hyde JE. 1990. The dihydrofolate reductase-thymidylate synthetase gene in the drug resistance of malaria parasites. Pharmacology and Therapeutics, 48(1), 45–59. [Google Scholar]
  8. Imwong M, Madmanee W, Suwannasin K, Kunasol C, Peto TJ, Tripura R, von Seidlein L, Nguon C, Davoeung C, Day NPJ, Dondorp AM, White NJ. 2019. Asymptomatic natural human infections with the simian malaria parasites Plasmodium cynomolgi and Plasmodium knowlesi. Journal of Infectious Diseases, 219(5), 695–702. [CrossRef] [PubMed] [Google Scholar]
  9. Imwong M, Pukrittakayamee S, Looareesuwan S, Pasvol G, Poirreiz J, White NJ, Snounou G. 2001. Association of genetic mutations in Plasmodium vivax dhfr with resistance to sulfadoxine-pyrimethamine: geographical and clinical correlates. Antimicrobial Agents and Chemotherapy, 45(11), 3122–3127. [Google Scholar]
  10. Imwong M, Pukrittayakamee S, Cheng Q, Moore C, Looareesuwan S, Snounou G, White NJ, Day NP. 2005. Limited polymorphism in the dihydropteroate synthetase gene (dhps) of Plasmodium vivax isolates from Thailand. Antimicrobial Agents and Chemotherapy, 49(10), 4393–4395. [Google Scholar]
  11. Japrung D, Leartsakulpanich U, Chusacultanachai S, Yuthavong Y. 2007. Conflicting requirements of Plasmodium falciparum dihydrofolate reductase mutations conferring resistance to pyrimethamine-WR99210 combination. Antimicrobial Agents and Chemotherapy, 51(12), 4356–4360. [Google Scholar]
  12. Jones G, Willett P, Glen RC, Leach AR, Taylor R. 1997. Development and validation of a genetic algorithm for flexible docking. Journal of Molecular Biology, 267(3), 727–748. [Google Scholar]
  13. Karnchaisri K, Day NPJ, Dondorp AM, Malaivijitnond S, Imwong M. 2024. Prevalence and genetic diversity of simian malaria in wild macaque populations across Thailand: Implications for human health. Acta Tropica, 254, 107187. [Google Scholar]
  14. Kojom Foko LP, Kumar A, Hawadak J, Singh V. 2023. Plasmodium cynomolgi in humans: current knowledge and future directions of an emerging zoonotic malaria parasite. Infection, 51(3), 623–640. [Google Scholar]
  15. Kongsaeree P, Khongsuk P, Leartsakulpanich U, Chitnumsub P, Tarnchompoo B, Walkinshaw MD, Yuthavong Y. 2005. Crystal structure of dihydrofolate reductase from Plasmodium vivax: pyrimethamine displacement linked with mutation-induced resistance. Proceedings of the National Academy of Sciences of the United States of America, 102(37), 13046–13051. [Google Scholar]
  16. Lee KS, Divis PC, Zakaria SK, Matusop A, Julin RA, Conway DJ, Cox-Singh J, Singh B. 2011. Plasmodium knowlesi: reservoir hosts and tracking the emergence in humans and macaques, PLOS Pathogens, 7(4), e1002015. [Google Scholar]
  17. Malde AK, Zuo L, Breeze M, Stroet M, Poger D, Nair PC, Oostenbrink C, Mark AE. 2011. An automated force field topology builder (ATB) and repository: version 1.0. Journal of chemical theory and computation, 7(12), 4026–4037. [Google Scholar]
  18. Mokmak W, Chunsrivirot S, Hannongbua S, Yuthavong Y, Tongsima S, Kamchonwongpaisan S. 2014. Molecular dynamics of interactions between rigid and flexible antifolates and dihydrofolate reductase from pyrimethamine-sensitive and pyrimethamine-resistant Plasmodium falciparum. Chemical Biology & Drug Design, 84(4), 450–461. [Google Scholar]
  19. Muehlenbein MP, Ancrenaz M, Sakong R, Ambu L, Prall S, Fuller G, Raghanti MA. 2012. Ape conservation physiology: fecal glucocorticoid responses in wild Pongo pygmaeus morio following human visitation. PLoS One, 7(3), e33357. [Google Scholar]
  20. Pasini EM, Bohme U, Rutledge GG, Voorberg-Van der Wel A, Sanders M, Berriman M, Kocken CH, Otto TD. 2017. An improved Plasmodium cynomolgi genome assembly reveals an unexpected methyltransferase gene expansion. Wellcome Open Research, 2, 42. [Google Scholar]
  21. Pornthanakasem W, Riangrungroj P, Chitnumsub P, Ittarat W, Kongkasuriyachai D, Uthaipibull C, Yuthavong Y, Leartsakulpanich U. 2016. Role of Plasmodium vivax dihydropteroate synthase polymorphisms in sulfa drug resistance. Antimicrobial Agents and Chemotherapy, 60(8), 4453–4463. [Google Scholar]
  22. Putaporntip C, Jongwutiwes S, Thongaree S, Seethamchai S, Grynberg P, Hughes AL. 2010. Ecology of malaria parasites infecting Southeast Asian macaques: evidence from cytochrome b sequences. Molecular Ecology, 19(16), 3466–3476. [CrossRef] [PubMed] [Google Scholar]
  23. Putaporntip C, Kuamsab N, Pattanawong U, Yanmanee S, Seethamchai S, Jongwutiwes S. 2021. Plasmodium cynomolgi co-infections among symptomatic malaria patients, Thailand. Emerging Infectious Diseases, 27(2), 590–593. [Google Scholar]
  24. Putaporntip C, Kuamsab N, Seethamchai S, Pattanawong U, Rojrung R, Yanmanee S, Cheng CW, Jongwutiwes S. 2021. Cryptic Plasmodium inui and P. fieldi infections among symptomatic malaria patients in Thailand. Clinical Infectious Diseases, 75(5): 805–812. [Google Scholar]
  25. R Core Team. 2023. R: A language and environment for statistical computing. Available at https://www.R-project.org/. [Google Scholar]
  26. Roth A, Maher SP, Conway AJ, Ubalee R, Chaumeau V, Andolina C, Kaba SA, Vantaux A, Bakowski MA, Thomson-Luque R, Adapa SR, Singh N, Barnes SJ, Cooper CA, Rouillier M, McNamara CW, Mikolajczak SA, Sather N, Witkowski B, Campo B, Kappe SHI, Lanar DE, Nosten F, Davidson S, Jiang RHY, Kyle DE, Adams JH. 2018. A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum. Nature Communications, 9(1), 1837. [Google Scholar]
  27. Sai-Ngam P, Pidtana K, Suida P, Poramathikul K, Lertsethtakarn P, Kuntawunginn W, Tadsaichol S, Arsanok M, Sornsakrin S, Chaisatit C, Mathavarat C, Thaloengsok S, Boonyarangka P, Thongpiam C, Demons S, Vesely B, Waters NC, Saejeng A, Wojnarski M, Tabprasit S, Kwanpichit C, Griesenbeck JS, Spring M. 2022. Case series of three malaria patients from Thailand infected with the simian parasite, Plasmodium cynomolgi. Malaria Journal, 21(1), 142. [Google Scholar]
  28. Schrödinger L. 2021. The PyMOL molecular graphics system. [Google Scholar]
  29. Sirawaraporn W, Sirawaraporn R, Yongkiettrakul S, Anuwatwora A, Rastelli G, Kamchonwongpaisan S, Yuthavong Y. 2002. Mutational analysis of Plasmodium falciparum dihydrofolate reductase: the role of aspartate 54 and phenylalanine 223 on catalytic activity and antifolate binding. Molecular and Biochemical Parasitology, 121(2), 185–193. [Google Scholar]
  30. Swanson JM, Henchman RH, McCammon JA. 2004. Revisiting free energy calculations: a theoretical connection to MM/PBSA and direct calculation of the association free energy. Biophysical Journal, 86(1 Pt 1): 67–74. [Google Scholar]
  31. Tamura K, Stecher G, Kumar S. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38(7), 3022–3027. [CrossRef] [PubMed] [Google Scholar]
  32. Thongdee P, Kuesap J, Rungsihirunrat K, Tippawangkosol P, Mungthin M, Na-Bangchang K. 2013. Distribution of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles in Plasmodium vivax isolates from Thailand. Acta Tropica, 128(1), 137–143. [Google Scholar]
  33. Triglia T, Cowman AF. 1994. Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparum. Proceedings of the National Academy of Sciences of the United States of America, 91(15), 7149–7153. [Google Scholar]
  34. Vanichtanankul J, Taweechai S, Uttamapinant C, Chitnumsub P, Vilaivan T, Yuthavong Y, Kamchonwongpaisan S. 2012. Combined spatial limitation around residues 16 and 108 of Plasmodium falciparum dihydrofolate reductase explains resistance to cycloguanil. Antimicrobial Agents and Chemotherapy, 56(7), 3928–3935. [Google Scholar]
  35. Wang P, Lee CS, Bayoumi R, Djimde A, Doumbo O, Swedberg G, Dao LD, Mshinda H, Tanner M, Watkins WM, Sims PF, Hyde JE. 1997. Resistance to antifolates in Plasmodium falciparum monitored by sequence analysis of dihydropteroate synthetase and dihydrofolate reductase alleles in a large number of field samples of diverse origins. Molecular and Biochemical Parasitology, 89(2), 161–177. [Google Scholar]
  36. Yogavel M, Nettleship JE, Sharma A, Harlos K, Jamwal A, Chaturvedi R, Sharma M, Jain V, Chhibber-Goel J, Sharma A. 2018. Structure of 6-hydroxymethyl-7, 8-dihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium vivax sheds light on drug resistance. Journal of Biological Chemistry, 293(39), 14962–14972. [Google Scholar]
  37. Yusuf NM, Zulkefli J, Jiram AI, Vythilingam I, Hisam S, Devi R, Salehhuddin A, Ali NM, Isa M, Alias N, Ogu Salim N, Aziz AA, Sulaiman LH. 2022. Plasmodium spp. in macaques, Macaca fascicularis, in Malaysia, and their potential role in zoonotic malaria transmission. Parasite, 29, 32. [Google Scholar]
  38. Yuthavong Y, Yuvaniyama J, Chitnumsub P, Vanichtanankul J, Chusacultanachai S, Tarnchompoo B, Vilaivan T, Kamchonwongpaisan S. 2005. Malarial (Plasmodium falciparum) dihydrofolate reductase-thymidylate synthase: structural basis for antifolate resistance and development of effective inhibitors. Parasitology, 130(Pt 3), 249–259. [Google Scholar]
  39. Yuvaniyama J, Chitnumsub P, Kamchonwongpaisan S, Vanichtanankul J, Sirawaraporn W, Taylor P, Walkinshaw MD, Yuthavong Y. 2003. Insights into antifolate resistance from malarial DHFR-TS structures. Nature Structural Biology, 10(5), 357–365. [Google Scholar]
  40. Zhang X, Kadir KA, Quintanilla-Zarinan LF, Villano J, Houghton P, Du H, Singh B, Smith DG. 2016. Distribution and prevalence of malaria parasites among long-tailed macaques (Macaca fascicularis) in regional populations across Southeast Asia. Malaria Journal, 15(1), 450. [Google Scholar]

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