Supplementary material

Supplementary Table S1. Raw data for calculation of infection rates of each Encephalitozoon species based on cell lines and biological replicate. Download File(s)

The total number of cells on the day of infection and 48 hours later is taken into account when calculating the infection rate. The infection rate is calculated by dividing the total number of infected cells at 48 hours post-infection by the number of viable cells at the time of infection. PI: post-infection; p25: 25th percentile; p75: 75th percentile; SD: standard deviation.

Supplementary Table S2. P-values for pairwise comparisons of infection rates between the six cell lines for each of the three Encephalitozoon species. Download File(s)

Regarding E. hellem, the overall p-value was not significant (p = 0.08), so pairwise comparisons were not carried out. Significant p-values (≤ 0.05) are shown in bold.

Supplementary Table S3. P-values for pairwise comparisons of the surface area of infectious foci between the six cell lines for each of the three Encephalitozoon species. Download File(s)

Significant p-values (≤ 0.05) are shown in bold.

© L. Vercruysse et al., published by EDP Sciences, 2026