Open Access
Issue
Parasite
Volume 33, 2026
Article Number 7
Number of page(s) 13
DOI https://doi.org/10.1051/parasite/2026004
Published online 11 February 2026

Supplementary material

Supplementary Table 1: Specific primers targeting ITS1 and pepck of liver fluke. Access here

Supplementary Table 2: Assessment guidelines for viability scoring. Access here

thumbnail Supplementary Figure 1:

Morphology and morphometry of eggs laid by aspermic Fasciola (asF) and spermic Fasciola gigantica (sFg). Adult flukes were incubated in DMEM supplemented with 20% bovine serum, 20% bovine bile along with 200 U/mL penicillin, and 200 mg/mL streptomycin in a 12-well flat bottom cell culture plate. Eggs were collected the following morning. (A) Microphotograph of the eggs laid by asF and sFg. (B) Size of eggs laid by asF and sFg.

thumbnail Supplementary Figure 2:

Venn diagram showing mixed infection with aspermic Fasciola (asF) and spermic Fasciola gigantica (sFg).


© M.H. Ali et al., published by EDP Sciences, 2026

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