Supplementary Material

miRNA expression levels for the data mapped to the miRBase 21 database of various known species showing the miRNA name (column A), the expression level (column B), and the miRNA sequence (column C). (Access here)

Differential expression analysis of the two tick species. Pairwise comparison results (column A), the miRNA name (column B), and the total reads in the two strains (columns C and D). The normal expression levels in the different strains (columns E and F). The expression levels of the normalized miRNAs (columns G and H). When HLCS was used as a control, the relative expression level with negative numbers, indicating down-regulation, and positive numbers, indicating up-regulation (column I). The p-value of the expression level, and the last column (*) indicates a significant difference (column J).

Note: the expression level of miRNA will be normalized to 0, and this value cannot be used as the dividend when calculating the difference in expression. Therefore, even if the p-value is very low, the difference is meaningless. It does not have to be marked as significant.

Novel miRNA structures were analyzed at different developmental stages. The long sequence indicates the miRNA precursor information in the following order: sequence, name and length. The parentheses indicate the miRNA precursor information in the following order: hairpin structure, structure and MFE (minimum free energy). *** and the short sequences indicate mature miRNA information in the following order: sequence, name and length; “…” and the short sequences indicate information for the matched sRNA tags in the following order: sequence, ID, length, and count. (Access here)

The stem-loop structure of all novel miRNAs in the HLCS ticks. (Access here)

The stem-loop structure of all novel miRNAs in the HLWS ticks. The structure was not provided for HLWS-m0016 and HLWS-m0032 with the sequencing. (Access here)

GO characteristics described for novel miRNAs. Accession numbers for GO and ontology terms and potential sites are provided for the target genes of novel miRNAs. The GO classification provides all analysis terms that are significantly enriched in the predicted target gene candidates of novel miRNAs compared to a reference gene background. (Access here)

© J. Luo et al., published by EDP Sciences, 2019