Surface areas of three species of Encephalitozoon in six cell lines. Measurement of the surface area of infectious foci within cells infected by E. intestinalis, E. hellem, and E. cuniculi at 48 h post-infection. The results were obtained from three biological replicates. The number of areas measured is indicated under each cell line. The median is represented by a solid line. The overall p-value is shown in parentheses for each species. Post hoc pairwise comparisons are indicated by asterisks: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.