Western blot of NF-κB pathway proteins in M1 polarized RAW264.7 macrophages. RAW264.7 macrophages were treated with rTsSPc, and the expression of NF-κB signaling pathway-related proteins was assessed using Western blot analysis. The relative intensity of protein signals for p-IKKβ, p-IκB-α, and p-NF-κB p65 was quantified, and β-actin was used as the internal control. Each test had three replicates. *Compared to the DMEM group, p < 0.05.