Glossina from the Republic of the Congo: species identification by MALDI-TOF MS and research of associated micro-organisms

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Table 1

Overview of molecular characterization results for Glossina species and subspecies using COI-1, COI-2, and ITS1 gene markers.

Morphological ID		COI-1			COI-2			ITS1
	Number per group (n)	Molecular ID	Accession number	Proportion of coverage/identity (range)	Molecular ID	Accession number	Proportion of coverage/identity (range)	Molecular ID	Accession number	Proportion of coverage/identity (range)
Glossina palpalis group	10^a	G. palpalis	EF531202	91–100% /97.07–97.80%	G. p. palpalis	MN750714	99–100%/99.47–100%	G. p. palpalis	LR813478	98–100%/97.69–98.98%
	48^b	G. palpalis	EF531202	91–100% /97.07–97.80%	G. p. palpalis	MN750714	99–100%/99.47–100%	G. p. palpalis	LR813478	98–100%/97.69–98.98%
Glossina fuscipes group	9^a	G. f. fuscipes	KP979583 KP979584	98–100%/99.22–99.86%	G. f. quanzensis	HQ387030	100%/100%	G. f. quanzensis	EU591941	100%/100%
	7^b	G. f. fuscipes	KP979583 KP979584	98–100%/99.22–99.86%	G. f. quanzensis	HQ387030	100%/100%	G. f. quanzensis	EU591941	100%/100%

Specimens used for the creation of the MALDI-TOF MS reference database.

Specimens obtaining discrepant results between morphological and MALDI-TOF MS identification.

ID, Identification; COI, cytochrome oxidase; ITS, internal transcribed spacer.

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