Figure 3
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The cytopathic effect on SIRC cells of Acanthamoeba pretreated with different water conditions. SIRC monolayers with adherent Acanthamoeba pretreated with PYG medium, PAS, domestic tap water, CaCl2, or DDW were incubated for 24 h. Following the 24 hour-infection period, the cell layers were analyzed using a ZOE Fluorescent Cell Imager (Bio-Rad).
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