Table 4
Reproducibility and intra- and inter-assay coefficients of variation.
| Batches | Parameters | PC | NC | Sample 1 (−) | Sample 2 (−) | Sample 3 (+) | Sample 4 (+) |
|---|---|---|---|---|---|---|---|
| Batch 1 | Mean OD ± SD | 2.186 ± 0.055 | 0.046 ± 0.002 | 0.057 ± 0.003 | 0.065 ± 0.003 | 2.211 ± 0.053 | 2.138 ± 0.043 |
| Minimum OD | 2.108 | 0.044 | 0.054 | 0.060 | 2.136 | 2.072 | |
| Maximum OD | 2.280 | 0.049 | 0.061 | 0.069 | 2.264 | 2.202 | |
| Intra-assay %CV | 2.498 | 4.374 | 4.437 | 4.817 | 2.419 | 1.997 | |
| Batch 2 | Mean OD | 2.023 ± 0.038 | 0.051 ± 0.003 | 0.056 ± 0.003 | 0.061 ± 0.003 | 2.000 ± 0.065 | 2.077 ± 0.044 |
| Minimum OD | 1.983 | 0.047 | 0.052 | 0.057 | 1.900 | 1.988 | |
| Maximum OD | 2.072 | 0.055 | 0.062 | 0.064 | 2.096 | 2.126 | |
| Intra-assay %CV | 1.865 | 5.377 | 5.570 | 5.075 | 3.252 | 2.097 | |
| Batch 3 | Mean OD | 2.084 ± 0.083 | 0.048 ± 0.003 | 0.056 ± 0.003 | 0.062 ± 0.003 | 2.083 ± 0.081 | 2.161 ± 0.079 |
| Minimum OD | 1.988 | 0.044 | 0.054 | 0.056 | 1.934 | 2.003 | |
| Maximum OD | 2.206 | 0.053 | 0.062 | 0.066 | 2.166 | 2.262 | |
| Intra-assay %CV | 3.971 | 6.106 | 5.524 | 5.049 | 3.906 | 3.675 | |
| Inter-assay %CV | 2.778 | 5.286 | 5.177 | 4.980 | 3.192 | 2.590 |
ELISA tests were performed in 8 replicates on 3 plate batches (24 tests for each sample). PC: positive control; NC: negative control. Samples: negatives (n = 1, n = 2), positives (n = 3, n = 4). OD: optical density; SD: standard deviation. CV: coefficient of variation.
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