Table 2
ELISA positive and negative controls evaluation.
| Samples | Dilution | Mean OD ± SD |
|---|---|---|
| PC1 | 1:1000 | 0.089 ± 0.000 (n = 2) |
| PC2 | 1:1000 | 0.105 ± 0.006 (n = 2) |
| 1:8000 | 0.058 ± 0.001 (n = 2) | |
| PC3 | 1:1000 | 0.127 ± 0.002 (n = 2) |
| 1:8000 | 0.069 ± 0.002 (n = 2) | |
| PC4 | 1:100 | 1.680 ± 0.088 (n = 16) |
| 1:150 | 1.426 ± 0.062 (n = 16) | |
| 1:200 | 1.279 ± 0.060 (n = 16) | |
| Negative samples | 1:100 | 0.083 ± 0.040 (n = 58) |
| NC | – | 0.051 ± 0.004 (n = 10) |
Three anti-His tag HRP antibodies (PC1, PC2, and PC3) were tested at dilutions 1:1000 and 1:8000. ELISA results are reported as mean OD ± standard deviation (SD). PC1: monoclonal anti-His HRP antibody (Sigma-Aldrich); PC2: polyclonal anti-His tag HRP antibody (Abcam Limited); PC3: monoclonal anti-His tag HRP antibody (Thermo Fisher Scientific); PC4: pool of canine positive sera (1:100, 1:150, 1:200); NC: negative control (sample diluent); n: number of tests.
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