Table 1
Preliminary ELISA test.
| Samples | IFAT | Conjugate 1:30,000 |
Conjugate 1:40,000 |
||
|---|---|---|---|---|---|
| 0.5 μg/mL | 1 μg/mL | 0.5 μg/mL | 1 μg/mL | ||
| Sample 1 | NEG | n.d. | n.d. | 0.129 ± 0.001 | 0.151 ± 0.004 |
| Sample 2 | NEG | n.d. | n.d. | 0.056 ± 0.001 | 0.059 ± 0.001 |
| Sample 3 | NEG | n.d. | n.d. | 0.082 ± 0.005 | 0.094 ± 0.004 |
| Sample 4 | NEG | n.d. | n.d. | 0.050 ± 0.001 | 0.049 ± 0.000 |
| Sample 5 | NEG | 0.057 ± 0.001 | 0.054 ± 0.000 | 0.056 ± 0.001 | 0.056 ± 0.002 |
| Sample 6 | NEG | 0.076 ± 0.004 | 0.074 ± 0.005 | 0.074 ± 0.001 | 0.068 ± 0.002 |
| Sample 7 | NEG | n.d. | n.d. | 0.070 ± 0.001 | 0.069 ± 0.000 |
| Sample 8 | NEG | n.d. | n.d. | 0.054 ± 0.001 | 0.052 ± 0.003 |
| Sample 9 | NEG | n.d. | n.d. | 0.057 ± 0.000 | 0.057 ± 0.003 |
| Sample 10 | NEG | n.d. | n.d. | 0.049 ± 0.003 | 0.057 ± 0.001 |
| Sample 11 | NEG | n.d. | n.d. | 0.054 ± 0.000 | 0.059 ± 0.000 |
| Sample 12 | NEG | n.d. | n.d. | 0.049 ± 0.001 | 0.047 ± 0.002 |
| Sample 13 | POS | 2.604 ± 0.050 | 2.533 ± 0.039 | 2.189 ± 0.025 | 2.332 ± 0.012 |
| Sample 14 | POS | 1.985 ± 0.023 | 1.843 ± 0.003 | 1.473 ± 0.006 | 1.554 ± 0.016 |
| Sample 15 | POS | 2.143 ± 0.016 | 2.215 ± 0.018 | 1.761 ± 0.018 | 1.793 ± 0.005 |
| Sample 16 | POS | 0.635 ± 0.000 | 0.638 ± 0.013 | 0.566 ± 0.040 | 0.657 ± 0.004 |
| Cut-off | 0.175 | 0.171 | 0.169 | 0.173 | |
Plates were coated with 0.5 μg/mL or 1 μg/mL of E. canis gp19 antigen. Results are reported as mean OD values ± standard deviation. Each sample was tested diluted 1:100 in duplicate, with the conjugate antibody at 1:30,000 and 1:40,000 dilutions. The cut-off was calculated as the mean OD value of negative samples plus 3 times standard deviation, considering positive or negative samples with OD values 10% above or below the cut-off, respectively. NEG: negative samples; POS: positive sample; n.d.: not determined.
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