Table 2
Comparison analysis of polymorphic alleles PcyDHFR and PcyDHPS. The bold blue text indicates different residues among three strains of P. cynomolgi. Bold text represents the polymorphic alleles PcyDHFR and PcyDHPS in this study.
| Equivalent residues in human malaria | P. cynomolgi strains | P. cynomolgi in this study | ||||||
|---|---|---|---|---|---|---|---|---|
| Available on database | Reference | Residue numbering based on the reference sequence strain M | Homo sapiens (n = 9) | Macaca fascicularis (n = 29) | ||||
| P. falciparum strain 3D7 | P. vivax strain SalI | Strain RO1 | Strain B2/strain M3 | Battambang, Cambodia |
Saraburi, Thailand |
Saraburi, Thailand |
||
| Haplotype 1 100% (9/9) | Haplotype 1 72.41% (21/29) | Haplotype 2 27.59% (8/29) | ||||||
| Active site of PcyDHFR [8, 13] | A16 | A15 | A15 | A | A15 | • | • | • |
| N51 | N50 | N50 | N | N50 | • | • | • | |
| F58 | F57 | F57 | F | F57 | • | • | • | |
| C59 | S58 | S58 | S | S58 | • | • | • | |
| S108 | S117 | S123 | S | S123 | • | • | • | |
| I164 | I173 | I179 | I | I179 | • | • | • | |
| PcyDHFR nonsynonymous mutations [8, 13] | V8 | V7 | – | I | I7 | • | • | V* |
| K23 | T22 | S22 | S | S22 | • | P | P | |
| N24 | S23 | N23 | N | N23 | • | S | S | |
| N34 | P33 | S33 | S | S33 | • | P | P | |
| V45 | T44 | N44 | N | N44 | • | T | T | |
| C50 | C49 | G49 | C | C49 | G | S | S | |
| Y80 | Y79 | Y79 | F | F79 | Y | Y | Y | |
| K83 | M82 | R82 | R | R82 | • | K | K | |
| – | D90 | D90 | D | D90 | • | G | G | |
| – | T92 | H92 | H | H92 | • | L | L | |
| – | G94 | G94 | G | G94 | • | S | S | |
| – | – | T97 | T | T97 | • | I | I | |
| – | – | N98 | N | N98 | • | S | S | |
| N88 | N97 | A103 | A | A103 | • | T | T | |
| D91 | G100 | T106 | T | T106 | • | I | I | |
| N94 | N103 | N109 | N | N109 | • | S | S | |
| S95 | A104 | N110 | N | N110 | • | T | T | |
| K133 | K142 | Y148 | Y | Y148 | • | H | H | |
| D135 | D144 | H150 | H | H150 | • | Q | Q | |
| Y141 | F150 | F156 | F | F156 | • | Y | Y | |
| E147 | D156 | D162 | G | G162 | D | D | D | |
| N157 | K166 | R172 | R | R172 | • | K | K | |
| Q171 | R180 | R186 | R | R186 | • | G | G | |
| T190 | A199 | A205 | S | S205 | A | A | A | |
| Active site of PcyDHPS | S436 | S382 | NA | S382 | S382 | • | • | • |
| A437 | A383 | NA | A383 | A383 | • | • | • | |
| K540 | K512 | NA | K512 | K512 | • | • | • | |
| A581 | A553 | NA | A553 | A553 | • | • | • | |
| A613 | V585 | NA | A585 | A585 | • | V | V | |
| PcyDHPS nonsynonymous mutations | D465 | K411 | NA | K411 | K411 | • | N | N |
| N468 | C414 | NA | S414 | S414 | • | R | R | |
| – | A424 | NA | A424 | A424 | • | D | D | |
| – | A433 | NA | G433 | G433 | • | A | A | |
| – | V444 | NA | L444 | L444 | • | V | V | |
| I479 | I451 | NA | V451 | V451 | I | I | I | |
| P511 | P483 | NA | A483 | A483 | • | E | E | |
| S525 | S497 | NA | T497 | T497 | • | S | S | |
Note: The strain M was referred to as a wild-type sequence in this context because this strain provides a complete sequence of the pcydhfr and pcydhps on the PlasmoDB database. 1Strain RO from Macaca mulatta isolated in 1960 has DHFR accession number AY639976.1, while the DHPS sequence is unavailable. 2Strain B from Macaca fascicularis isolated in 1959 has accession number NC_020398.1 for DHFR, and NC_020407.1 for DHPS. 3Strain M from Macaca fascicularis isolated in 1933 has gene number PcyM_0526900 for DHFR, and NC_020407.1 for DHPS on the PlasmoDB database. • Indicates identical amino acid residues to the wild-type sequence. *Containing a mixed genotype changed nucleotide from ATA to A/GTA (n = 2 samples).
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.