(A) An overlay of the PcyDHPS models of 3 variants: wild-type (light grey), human (light orange), and macaque (pale cyan) isolates. The identified mutations in the study are depicted in a red ball and stick; a small box was zoomed in to view mutations in the region of loop-1. The dashed line indicates loop structures, two long insertion residues in P. cynomolgi, and a disordered PfDHPS template. The bound substrates pABA (yellow) and PtPP (green) are displayed in a ball and stick representation, while the cofactor magnesium is represented by an orange sphere. (B) Schematic protein sequence alignment of PcyDHPS against PfDHPS, which identified the two insertion sites in the PcyDHPS protein sequence (yellow) and disordered PfDHPS template structure (soft red) [2]. A dash indicates a gap in sequence alignment.