Table 4
Evaluation results of the multiplex qPCR assay compared to the HSP70-I-3′-UTR PCR assay for identification of L. martiniquensis and L. orientalis and detection of trypanosomatid.
| Target | Multiplex qPCR | HSP70-I-3′-UTR PCR |
Total | ||
|---|---|---|---|---|---|
| Positive (TPa) | Negative (TNb) | ||||
| L. martiniquensis | Positive (TP) | 42 | 0 | 42 | ePPV = TP/(TP + FP) = 100% |
| Negative (TN) | 0 | 27 | 27 | fNPV = TN/(TN + FN) = 100% | |
| Total | 42 | 27 | 69 | Kappa = 1.0 | |
| Sensitivity = TP/(TP + FNc) = 100% | Specificity = TN/(TN + FPd) = 100% | ||||
| L. orientalis | Positive (TP) | 2 | 0 | 2 | PPV = 100% |
| Negative (TN) | 0 | 67 | 67 | NPV = 100% | |
| Total | 2 | 67 | 69 | Kappa = 1.0 | |
| Sensitivity = 100% | Specificity = 100% | ||||
| Trypanosomatids | Positive (TP) | 44 | 0 | 44 | PPV = 100% |
| Negative (TN) | 0 | 25 | 25 | NPV = 100% | |
| Total | 44 | 25 | 69 | Kappa = 1.0 | |
| Sensitivity = 100% | Specificity = 100% | ||||
aTP = true positive; bTN = true negative; cFN = false negative = 0; dFP = false positive = 0; ePPV = positive predictive value; fNPV = negative predictive value.
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