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Limit of detection (LOD95) of the multiplex qPCR for (A) L. martiniquensis, (B) L. orientalis, and (C) Crithidia sp. (CLA-KP1) was estimated by fitting a logistic regression model with detection results. Each dot indicates an actual detection rate at each dilution. The dashed line in the figure indicates the dilution ratio corresponding to a 95% probability.
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