File S3.

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PCR products of the ITS1 fragment of 149 bp using ITS1-L. mar primers, the ITS1 fragment of 146 bp using ITS1-L. ori/cha primers, and the ITS1 conserved fragment of 77 bp using ITS1-Tryps primers. Lanes: MW = Molecular weight markers; 1 = Negative control; 2 = human DNA; 3 = L. martiniquensis; 4 = L. orientalis; 5 = L. chancei; 6 = L. infantum; 7 = L. braziliensis; 8 = Crithidia sp. (CLA-KP1); 9 = L. martiniquensis + human DNA; 10 = L. orientalis + human DNA.
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