A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples

Open Access

Figure 1

Alignment of the conserved regions using Crustal analysis of (A) ITS1 target for Leishmania martiniquensis (green boxes) and ITS1 target for L. orientalis/L. chancei (blue boxes) and (B) ITS1 target for trypanosomatids (red boxes) used to design primers (F, R) and probes (Prob).

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