Open Access
Table 1
Primers used in the characterization of E. bieneusi and Blastocystis sp.
| Gene | Primer (5′–3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| SSU rRNA of Blastocystis sp. | SSU rRNA-F: ATCTGGTTGATCCTGCCAGT | 600 | 55 |
| SSU rRNA-R: GAGCTTTTTAACTGCAACAACG | |||
| ITS gene of E. bieneusi | ITS-F1: GGTCATAGGGATGAAGAG | 392 | 57 |
| ITS-R1: TTCGAGTTCTTTCGCGCTC | |||
| ITS-F2: GCTCTGAATATCTATGGCT | 55 | ||
| ITS-R2: ATCGCCGACGGATCCAAGTG |
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