Neighbor-joining tree of E. bieneusi ITS genotypes. Phylogenetic relationships of ITS nucleotide sequences of the E. bieneusi genotypes identified in this study and other reported genotypes. The phylogeny was inferred by a neighbor-joining analysis. Bootstrap values were obtained using 1000 replicates; those with values >50% are shown on the nodes. The genotypes in this study are marked by empty circles, and the novel genotypes are marked by filled circles.