Phylogenetic analysis of trichomonads based on the ITS1-5.8S rRNA-ITS2 gene. Sequences were retrieved from GenBank, aligned using ClustalW, and analyzed using MEGA 11 software. The neighbor-joining method was used to construct the trees from the Kimura-2-parameter model. Branch numbers represent percent bootstrapping values from 1,000 replicates, with values of more than 60% shown in the tree. The species identified in this study are indicated by ▲.