Validation of edition AQP and PAPO-I knock-out. Upper panels: schematic representation of each gene locus and primers (black arrows) used to confirm integration of the different drug resistant markers and loss of WT allele in the respective cell lines. Geneticin (GENET); puromycin (PURO). Wells: L is GeneRuler^TM 1 kb Plus DNA ladder, wells 1 to 6 represent the different primer pairs used to check for edition. [A] LdAQP KO; 1: AL44/AL45, no PCR amplification in edited and a positive band at 660 bp in non-edited cells, 2: AL46/AL47: Positive PCR amplification at either 1 959 bp (integration of geneticin resistance) or 2 056 bp (puromycin), or 1 239 bp (non-edited), 3: AL46/AG07: 1 137 bp (edited) and negative for non-edited, 4: AG06/AL47: 1 268 bp (edited) and negative for non-edited, 5: AL46/AF89: 1 114 bp (edited) and negative for non-edited, 6: AF88/AL47: 1 409 bp (edited) and negative for non-edited. [B] LdPAPO-I KO; 1: AM39/AM40, negative in edited and positive at 23 bp in non-edited, 2: AM41/AM42: Positive with 2 014/2 111 bp (edited – geneticin/puromycin) and 2 986 bp (non-edited), 3: AM41/AG07: 1 196 bp (edited) and negative for non-edited, 4: AG06/AM42: 1 263 bp (edited) and negative for non-edited; 5: AM41/AF89: 1 173 bp (edited) and negative for non-edited, 6: AF88/AM42: 1 405 bp (edited) and negative for non-edited.