In vitro methods for the evaluation of anti-Echinococcus multilocularis compounds. In vitro methods are based on the exposure of protoscoleces, metacestodes, or cultures of germinal cells to compounds. Different techniques are used for the assessment of drug activity, including morphology and/or exclusion tests, phosphoglucoisomerase (PGI) and the Echinococcus multilocularis Alkaline Phosphatase (EmAP) assays, Em14-3-3 RT-qPCR, the Alamar Blue assay, and ATP quantification. Except for the first one, all of them rely on automated quantification of a signal. However, correlation of these signals with parasite viability remains difficult to predict.