Detection of the binding activity of recombinant CpLTL to mannose and the surface of host cells, and exploration of the efficiency of recombinant CpLTL protein and mannose on invasion. (A) Binding ability of GST-CpLTL to mannose was detected by ELISA. GST was used as an internal control for data normalization. (B, C) The binding activity of GST-CpLTL to the surface of fixed and live host cells was detected by ELISA. GST was used as an internal control for data normalization. (D) The binding activity of GST-CpLTL to the surface of fixed host cells was detected by IFA. Fixed host cells were incubated with GST-CpLTL or GST alone, labelled with anti-GST antibody and Alexa Fluor^® 594-labelled secondary antibody. (E) The inhibition efficiency of GST-CpLTL to invasion of C. parvum was quantified by qRT-PCR. Host cells were pre-incubated with different dilutions of GST-CpLTL and GST, with medium alone as a control. Data are presented as Mean ± SD from three replicate assays (p < 0.05). (F). The inhibition efficiency of mannose to invasion of C. parvum was quantified by qRT-PCR. Oocysts were pre-incubated with different dilutions of mannose and PBS, with medium alone as a control. Data are presented as Mean ± SD from three replicate assays (p < 0.05).