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All issues Volume 29 (2022) Parasite, 29 (2022) 47 Full HTML
Open Access
Review
Issue
Parasite
Volume 29, 2022
Article Number 47
Number of page(s) 15
DOI https://doi.org/10.1051/parasite/2022047
Published online 21 October 2022

© Z. Nasiri et al., published by EDP Sciences, 2022

Licence Creative CommonsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction

Phlebotomus and Lutzomyia sandflies have been shown to transmit Leishmania promastigotes through skin bites in vertebrate hosts in the Old and New World, respectively. Leishmania major, L. infantum, L. tropica, L. aethiopica, and L. donovani have been demonstrated to be mainly responsible for transmission of cutaneous leishmaniasis (CL) in the Old World [97, 102, 129].

Around 12 countries in Asia, Africa, and Latin America such as Iran, Afghanistan, Pakistan, Saudi Arabia, Syria, Turkey, Algeria, Morocco, Tunisia, Colombia, Peru, and Brazil were reported as engaged states against CL [145]. From March to September 2014, 3684 cases of CL were recorded in Iran, including in Khorasan-e-Razavi (Northeast), Fars (South), and Kerman (southeast) as the most endemic regions for CL, while 31% of affected patients were below the age of 14 years [38, 72, 126].

The annual incidence of the CL from 1983 to 2013 was 30.9 per 100,000, with spread in areas with dry and desert climates in particular in the central zone of Iran [52]. These areas are plains and Phlebotomus papatasi is the main vector reported there [27]. Rhombomys opimus was illustrated as the principal host of zoonotic cutaneous leishmaniasis (ZCL) in the center and northeast of the country, Meriones libycus as the main vector in the south, Meriones hurrianae as the major host in the southeast, and Tatera indica as the most prevalent host in the west and south of Iran [58, 83]. Strict strategies have been undertaken to control CL in Iran, but new foci have emerged in the center, northeast, and west of Iran. Therefore, the aim of this systematic review was to define the geographical distribution of Leishmania spp. among human populations as well as to determine methods used for identifying Leishmania parasites, reservoirs, and vector hosts and the impact of environmental factors in Iran over four decades (1983–2021) to help promote interventions against CL and to decrease the disease transmission rate.

Materials and methods

This systematic review has 5 sections including epidemiology of CL, reservoirs, vectors, climatic factors, and identification methods of Leishmania parasites in Iran. The authors used the preferred reporting items for systematic reviews and meta-analyses (PRISMA) standard guideline to carry out the review process and report findings.

Search strategy and selection criteria

In this study, all related published articles were studied from 1980 up to the end of 2021. The authors searched the databases including Web of Science, Scopus, PubMed, Springer, ResearchGate, Wiley Online, Ovid, Ebsco, Cochrane Library, Google Scholar, and SID for medical subject headings (MeSH) and relevant keywords such as cutaneous leishmaniasis, epidemiology, zoonotic cutaneous leishmaniasis (ZCL), anthroponotic cutaneous leishmaniasis (ACL), Leishmania major, Leishmania tropica, Iran, rodent, P. papatasi, sandfly, detection, PCR, the kinetoplast, ITS, parasite, reservoir, vector, Gerbillinae, Giemsa stain, and temperature. The authors used these keywords alone or in combination through the Boolean method.

Inclusion and exclusion criteria

All high-quality English and Persian language articles published in the world on the epidemiology of CL, reservoirs, vectors, climatic factors, and identification methods of Leishmania parasite in Iran were included in the study. The authors excluded articles of low quality, studies conducted in other countries of the world, review studies, meta-analyses, case reports, or series of cases.

Quality assessment

We assessed the quality of the articles using the Strobe checklist (strengthening the reporting of observational studies in epidemiology). This checklist has 22 parts that are scored based on the importance of each section, the minimum score is 15 and the maximum is 33. In this study, the score 20 is considered acceptable [147].

Screening and data extraction

The search results were imported into Endnote software v.x8-1 and duplicate titles were deleted. The authors entered selected studies into an abstract reading phase and checked them against the inclusion criteria. Then, the most relevant studies were selected for independent full-text reading by two researchers. The authors used a checklist to extract data from the selected studies in terms of the study location, study years, reservoir species, parasite species, DNA, and recognized method.

Selection of articles

Searching databases, the authors extracted 786 studies. First, the articles were entered into Endnote software and after an initial review, 181 articles were removed from the study due to duplication. Then, by reviewing the titles and abstracts of articles, 459 irrelevant articles were removed and after reviewing the full text of articles, 40 articles were excluded since they investigated other pathogens. Finally, 106 articles met the inclusion criteria and entered the process of systematic review (Fig. 1).

thumbnail Fig. 1

The PRISMA flow diagram.

Results and discussion

Geographic distribution of CL

Cutaneous leishmaniasis infection has been found in all 31 provinces of Iran.

The prevalence of ZCL is high in Khorasan, Fars, and Isfahan and the highest frequency of cases has been reported from Ilam, Bushehr, and Yazd, recently. Ahvaz city in Khuzestan province and Kerman and Bam cities in Kerman province are the high endemic foci of ACL in Iran. Overall, the highest and the lowest incidence rates of leishmaniasis are reported from the western and northwest parts of the country, respectively. Two forms of ZCL and ACL occurred due to L. major and L. tropica in Iran. Fars is known as an endemic focus of CL and Nesokia indica or M. libycuserythrourus are confirmed as reservoir of ZCL [75].

Isfahan province has been a focus of CL in recent decades. Moreover, some new foci have been added to the endemic areas listed in this province. In this province, from 2011 to 2016, Isfahan, Ardestan, and Kashan cities had a high burden of CL. Kashan has been reported as the primary focus for CL in Isfahan province, while children were affected a long time ago [87, 143]. In the Aran and Bidgol districts of Kashan, 1–9-year-old children have been the target of the CL parasite [111]. In Isfahan province, the northern parts such as Borkhar and Natanz are known as hyperendemic spots and teenagers are the predominant age group affected by the diseases [59].

Yazd province is another infected region adjacent to Isfahan province in central Iran. In this province, Khatam, Yazd, and Bafgh were reported as the most problematic counties of CL [20]. Qom province has also been demonstrated as another endemic region of CL. In 2009, an outbreak was reported in this area in particular in suburban locations: Ghomrood and Ghanavat. This province is a destination for immigrants and pilgrims, but indigenous transmission is stable in this area. The majority of patients are housekeepers and cases below the age of 25 years were more commonly reported in this area [125].

In Semnan province, Damghan is an important city in central Iran concerning CL, while villages in Damghan were reported as a vulnerable area for emergence of CL disease. Most of the cases in this region did not have a previous history of traveling to endemic areas [89]. In Tehran province, CL has spread among the student population [22].

Many new CL cases are imported to different provinces of Iran from endemic areas of Pakistan and Afghanistan that are believed to be the primary source of outbreaks in some parts of the country, such as Yazd, Tehran, and Fars provinces [75]. Furthermore, in Varamin and Pakdasht, endemic districts of CL, several cases were found to be Afghani patients above the age of 20 years [22]. In Tehran province, in Pakdasht city, in Fars province, and in the south of Iran, CL is approximately distributed in all cities and towns and most frequently in Shiraz, Firouz Abad, Ghirokarzin, Farashband, Larestan, Fasa, Jahrom, Arsanjan, Kherameh, Lamerd, Zarrin Dasht, and Marvdasht [6–8, 55, 56, 74–76, 99, 113]. In this zone, all age groups were affected with CL. Also, Afghani and Pakistani immigrants comprised large parts of the cases in the south of Fars province [21].

Khuzestan province is also recognized as a hub for CL in the southwest of Iran and as a newly discovered focus. In 2014, emergence of CL was reported in Ahvaz city [70]. Moreover, this disease affected farmers and students more than any other group in the community. Susangerd, Shushtar, Behbahan, Abadan, Khorramshahr, Shush, and Shadegan cities were well documented in this province [36, 45, 63–66, 141]. In Bushehr province, southwest of Iran, the incidence of CL was 1.7 per 10,000 in Dashtestan town located in the east of Bushehr city in the winter and autumn and in September and January in 2013 and 2014 [100]. Genaveh is the other focus of CL in Bushehr province and CL is prevalent in rural areas in this city. In Dashtestan, urban areas have been at risk more than other parts [61].

In Kerman province, a comprehensive study conducted by Sharifi et al. showed that Bam, Kerman, and Jiroft cities were the high-risk areas in the southeast of Iran [136]. Orzoieh district is one of the distinct endemic areas in this province as well [77]. Hormozgan province, in the south of Iran, has been affected by malaria much more than CL; therefore, insufficient comprehensive studies have been conducted on CL in this area. Jask County in the province is confirmed as a hot spot of CL and 90% of patients were from rural localities, with an incidence of 162.5 per 100,000 population from 2006 to 2009 [37]. Another survey showed that CL was mostly spread in the southeast and northwest of Hormozgan province [4, 26]. In Sistan and Baluchestan province, southeastern Iran, a few investigations have been performed on CL. Briefly, the results revealed that CL prevalence was higher in rural (51.7%) than in urbans areas (48.3%). Cities of Zahedan (87 cases), Mirjaveh (34 cases) and Chabahar (29 cases) had the highest infection rates and Fenouj (one patient) and Nikshahr (two patients) had the lowest rates of infection. Statistical analyses showed a significant relationship between living area and the disease, and this is probably due to the presence of malaria in the province. Zahedan, Mirjaveh, and Chabahar cities in this province were infected more than any other area, and the trend of disease declined from 2009 to 2014; mostly in children below the age of 10 years [122, 132].

In Khorasan Razavi province in northeast Iran, CL is prevalent in Torghabeh–Shandiz, Sarakhs, Daregaz, and Neyshabur cities and children in these areas are affected with the disease [71, 130]. In Khorasan Razavi, Sabzevar city, children below the age of 4 years in rural areas were demonstrated to have a greater incidence of CL [151]. Like Khorasan Razavi, North Khorasan province was a hot spot of CL in the northeast of the country including Esfarayen city as a challenging region with the age group of ≥15 years most affected [110]. In this province, Jajarm town has been the main spot of CL before 2006 [9]. In Golestan province, some parts such as Kalaleh town have been confirmed as significant sites of CL. Also, in this province, the disease is prevalent in Gonbad-e-Qabus City in children below the age of five years [103]. Another city in the province is Maraveh Tappeh which is located near Iran’s northern border with Turkmenistan and is one of the hot spots of CL [28]. The north of Iran has not been confirmed as an endemic area of CL, but it can be considered an area of risk because people from neighboring provinces mostly travel to these areas [94].

In the western part of Iran, different patterns of CL have been observed. Ilam province is adjacent to Khuzestan province and is a major endemic area [91]. Ilam, Dehloran, and Mehran cities which have a common border with Iraq have a remarkably susceptible condition concerning CL because many visitors move between Iran and Iraq through the Mehran border [91, 133]. Hamedan province is another region affected by CL with an unsteady incidence rate of 1.5 per 100,000 in 2008 which increased to 12.6 per 100,000 in 2015, since the local population (about 87.1%) frequently travelled to endemic areas of CL, mainly including Ilam, Khuzestan, and Isfahan provinces. Notably, the working-age group is the main infected population. In Hamadan province, Hamadan, Bahar, and Kaboudar-Ahang cities are the main CL-infected areas [5].

In Kermanshah province, in the west of Iran, a review by Hamzavi and Khademi from 1991 to 2012 showed that the incidence rate had dramatically increased from 1.5 per 100,000 in the 1990s to 7.4 per 100,000 in 2011–2012. Also, half of the cases reported traveling to endemic areas. Qasr-e-Shirin, Eslam Abad, and Sarpol-e-zahab cities in the province are prominent foci of CL [48]. Housewives were infected in Qasr-e-Shirin city more than any other group in the community [107]. In Lorestan Province, CL has been distributed over large rural areas of Poledokhtar city in the west of Iran [10]. More investigations are needed to elucidate all aspects of CL in this province. In south Khorasan Province, Birjand and Khoosf cities located in the east of Iran were found as foci of CL with an 80% history of traveling to endemic areas [2].

Reservoirs of CL

In Iran, several studies were carried out on host species of Leishmania parasites. In the central and northeastern regions of Iran, Rhombomys opimus (the Great gerbil) is more predominant [44, 118]. Rhombomys opimus has two predominant subspecies. In Golestan province, in the northwest of Iran, R. opimus sodalist subspecies has been reported; but in Isfahan, Semnan, Esfarayen, and Shirvan (northeast) cities, R. opimus sargadensis was illustrated as the prevalent subspecies [1]. In the Turkmen Sahra area, in the northeast of Iran, R. opimus was present with co-infection of L. major and L. turanica [86]. Also, in Golestan (northeast) and Esfahan (central) provinces, mixed infection by these parasites has been demonstrated in R. opimus [7].

In Isfahan city in central Iran, L. major zymodeme MON-26 was detected in Meriones libycus. It is considered an alternative host in this district [128, 149]. Additionally, in Qom province, central Iran, this species is the principal host for L. major in Qomrood city as an endemic area [152]. Perhaps in some parts of the northeast of Iran, such as Gonbad-e Qabus city; M. libycus is the main reservoir of ZCL [106]. Meriones lybicus is an absolute host for Leishmania amastigote in the south of Iran and M. persicus is a reservoir in some parts of this region as well. Notably, Leishmania amastigotes were detected in M. persicus for the first time in Iranian Azerbaijan in the northwest of the country [82, 85, 115–117]. In Fars province, in the south of Iran, M. libycus, T. indica and Gerbillus spp. were shown to be infected with L. major in Marvdash, Estahban, and Larestan cities [83, 84]. In Fars province, L. major has been isolated and detected by PCR assay in Mus musculus [104]. Also in this province, Rattus norvegicus has been reported as a potential host for L. major using isoenzyme electrophoresis and nested PCR [96]. In Jahrom, Fars Province, R. rattus has been shown as a reservoir for L. major [96].

In Hormozgan province, in the south of Iran, Gerbillus nanus (Muridae) and M. hurrianae have been possible reservoirs of ZCL [15]. In Sistan and Baluchestan province, in the southeast of Iran, M. hurrianae and T. indica were found to be important hosts for L. major [62]. In Bahreman city, in Kerman province in the southeast of Iran, R. opimus was reported as a major reservoir for ZCL [40]. In Mehran city in Ilam province, in the west of Iran, T. indica was documented as a predominant reservoir for ZCL in rural areas [57, 95]; but in Khuzestan province, in the southwest of Iran, other rodents are responsible for L. major transmission [90]. In Isfahan province and rural areas of the Damghan city in Semnan province, in central Iran, Nesokia indica was recognized as a probable reservoir of ZCL [25, 108]. In Iran, R. opimus, M. lybicus, and T. indica are generally well-documented species for the Leishmania parasite [14, 88, 119, 138] (Table 1, Fig. 2).

thumbnail Fig. 2

Distribution of cutaneous leishmaniasis reservoirs in Iran; 1: Meriones libycus, 2: Rhombomys opimus, 3: Gerbillus nanus, 4: Tatera indica, 5: Meriones persicus, 6: Nesokia indica, 7: Rattus norvegicus, 8: Meriones hurrianae, 9: Mus musculus, 10: Rattus rattus.

Table 1

Reservoirs of cutaneous leishmaniasis in Iran.

Cutaneous leishmaniasis vectors

Like studies about reservoirs, there are many published articles on Leishmania vectors in Iran. The fauna of Iranian sandflies was first reported in 1930 [154]. Phlebotomus is known as the principal vector of CL in Iran. Phlebotomus includes 6 subgenera; i.e., subgenus Adlerius, Euphlebotomus, Larroussius, Paraphlebotomus, Phlebotomus, and Synphlebotomus [60]. Natural infections with L. major were shown in P. (Phl.) papatasi in all ZCL spots of Iran and was reported as the principal vector [13].

Other Phlebotomus species are considered probable vectors: P. (Phl.) salehi has been reported in Jask, in Sistan and Baluchestan province in the south of Iran, P. (Syn.) ansarii in Isfahan, and Yazd provinces, P. caucasicus in Isfahan, Damghan, Sarbisheh, Zirkouh, Yazd, and Fasa cities, P. mongoliensis in Isfahan, Ilam, and Fasa cities, P. andrejevi in Isfahan, P. alexandri in Khuzestan province, Zirkouh, and Fasa cities, and P. (Phl.) bergeroti in Fars province. P. (Par.) sergenti was demonstrated to be responsible for ACL in Khorasan-e Razavi, Isfahan, Yazd, Kerman, Fars, and Tehran provinces and in Zirkouh as well [16, 18, 54, 67, 68, 105, 114, 142, 148, 150] (Table 2, Figs. 3 and 4).

thumbnail Fig. 3

Distribution of anthroponotic cutaneous leishmaniasis in vectors in Iran; A: Phlebotomus papatasi, B: Phlebotomus Salehi, C: Phlebotomus sergenti, D: Phlebotomus caucasicus, E: Phlebotomus mongolensis, F: Phlebotomus anderjevi, I: Phlebotomus ansarii, J: Phlebotomus alexandri, K: Phlebotomus perfiliewi, L: Phlebotomus jacusieli, M: Sergentomyia dentata, N: Sergentomyia sintoni, O: Sergentomyia clydei.

thumbnail Fig. 4

Distribution of zoonotic cutaneous leishmaniasis in vectors in Iran; 1: Phlebotomus papatasi, 2: Phlebotomus Salehi, 3: Phlebotomus sergenti, 4: Phlebotomus caucasicus, 5: Phlebotomus mongolensis, 6: Phlebotomus ansarii, 7: Phlebotomus alexandri, 8: Sergentomyia dentata, 9: Phlebotomus anderjevi, 10: Phlebotomus tobbi, 11: Sergentomyia theodori, 12: Sergentomyia clydei, 13: Sergentomyia Baghdadis, 14: Sergentomyia squamipleuris, 15: Phlebotomus bergeroti, 16: Sergentomyia sintoni, 17: Sergentomyia tiberiadis, 18: Phlebotomus jacusieli.

Table 2

Vectors of anthroponotic cutaneous leishmaniasis (ACL) and zoonotic cutaneous leishmaniasis (ZCL) Iran.

Effects of meteorological and ecological characteristics on CL

Control and surveillance of leishmaniasis are not possible unless all environmental factors are measured together with the identification of reservoirs and vectors. Therefore, systems such as GIS have been designed to predict incidence based on spatial and temporal components of CL. Climatic factors (temperature, precipitation, and relative humidity) can establish a different pattern of CL occurrence in two nearby cities such as Gilan-e-Gharb and Kermanshah in the west of Iran [43]. Temperature had a significant relationship with the incidence rate of CL in Isfahan province: sandflies were more active in dry or moderate seasons [112]. Thus, most cases in Iran are detected from September to December [8, 74]. During these months, the temperature reaches 23–27 °C, which is suitable for the reproduction of sandflies. On the other hand, emergence of sandfly species in hot months affects workers who have seasonal occupations [20].

On the contrary, in the west of Iran, some peaks of CL incidence have been shown in winter, reported in January and February after an incubation period of the disease [63, 64]. The burden of CL has different dynamics in Iran. In the northeast of Iran, rainfalls and floods demolish the breeding sites of sandflies; leading to a drop in CL [139]. In contrast, in the southwest of Iran, wet days and humidity significantly increase the incidence of CL cases [12]. CL cases were mostly found in low-altitude areas in the south, center, northeast, and west of Iran [93]. For example, in Mehran and Dehloran (Ilam province), CL is strictly spread at an elevation of fewer than 215 m [153].

Some disasters such as an earthquake can change sleeping patterns and increase CL incidence because people have to stay outside in an open space. This model was presented in Dehloran city, in the west of Iran, after the occurrence of an earthquake in 2015 [98]. Jagin Dam provided suitable ecological conditions for T. indica and P. papatasi due to agricultural activities [37]. Notably, Rhombomys opimus (great gerbil) as a diurnal rodent spends 2/3 of its time outside of the burrow for feeding and this behavior can cause intensive close contact with vectors. Another factor is vegetation, which leads CL to be more prevalent in landscapes with low vegetation cover in the northeast of Iran [92].

Leishmania parasite identification methods

Leishmania species are responsible for the spectrum of clinical manifestations or different epidemiologic features; thereby, specific detection of parasites is necessary to understand their complexity. Staining smears by Giemsa under microscope and cultivation were the common diagnostic tests for early detection for a long time. The sensitivity of parasitological diagnostic tests is less than 70%, which makes it necessary to have access to a large number of parasites in biopsy and experienced personnel. In contrast, the culture of promastigotes is a prolonged protocol and contamination is a concerning issue, but both are weak in the identification of Leishmania species [47].

Serological tests are not used extensively in CL diagnosis as their specificity and sensitivity fluctuate and have cross-reaction with trypanosomatid parasites [121]. The isoenzyme electrophoresis (IE) technique is considered the gold-standard method for the identification of Leishmania [101]. Molecular methods such as PCR are still used to detect the specific DNA of Leishmania and this is a well-founded source for identifying Leishmania species. DNA fragments such as the small subunit rRNA (SSU rRNA) gene, repetitive sequences, microsatellite DNA, splice leader mini-exon (SLME), the beta-tubulin gene region, the gp63 gene locus, mini-exon-derived RNA genes, internal transcribed spacer (ITS) regions of the rRNA genes, and kinetoplast DNA (kDNA), SLME, kDNA, and ITS1 are used in PCR assays for the diagnosis of Leishmania parasites isolated from microscopic and culture samples. As a result, the kDNA PCR is the best diagnostic method in terms of sensitivity for L. major and L. tropica [24, 41, 81, 124]. Different studies about Leishmania identification methods in human cases carried out in Iran are summarized in Table 3.

Table 3

Different Leishmania identification methods in human cases carried out in Iran.

Conclusion

Our findings showed that cutaneous leishmaniasis has various patterns in Iran. There is heterogeneity in leishmaniasis in different parts of the country based on molecular identifications. KDNA and ITS1 fragments have mostly been used in genomic diagnostic procedures, while KDNA is a favorite targeted region for amplifying by PCR. Travel has been a hallmark of the distribution of Leishmania in Hamedan and Ilam (in the west) and South Khorasan provinces of Iran. In Iran, R. opimus, M. lybicus, and T. indica were well-documented species for hosting the Leishmania parasites. In Khorasan Razavi province, Kerman, Yazd, and sometimes in Shiraz and Tehran cities, L. major is present, but L. tropica is the predominant parasite.

The prevalence of R. opimus extends from Turkmen Sahra in the northeast to Isfahan in center of Iran with co-infection of L. major and L. turanica. More studies are required to clarify the potential role of L. turanica in CL. Furthermore, L. gerbilli has been identified in some vectors, such as P. papatasi, P. sergenti, P. caucasicus, P. mongoliensis, P. andrejevi, and P. ansarii. The relationship schema between vectors and reservoirs is complicated. Some sandflies play the role of common vectors concerning different parasite species. Although cutaneous leishmaniasis has been a predicament for the health system, it is generally controlled in Iran. Spraying, insecticide-treated nets (ITNs), and improvement in social services in the suburban community can reduce the problems associated with CL.

Conflict of interest

The authors declare no conflict of interest.

Acknowledgments

The authors thank the Research Vice-chancellor of Shiraz University of Medical Sciences.

a

These authors contributed equally to this work (Co-first author).

References

  1. Abai MR, Oshaghi MA, Tajedin L, Rassi Y, Akhavan AA. 2010. Geographical distribution and ecological features of the great gerbil subspecies in the main zoonotic cutaneous leishmaniasis foci in Iran. Asian Pacific Journal of Tropical Medicine, 3(10), 800–803. [CrossRef] [Google Scholar]
  2. Abdollahzadeh R, Shayesteh M. 2018. Epidemiological study of cutaneous leishmaniasis and factors affecting it in South Khorasan Province, 1391–1395 (2012–2016). Journal of Jahrom University of Medical Sciences, 16(1), 59–66. [Google Scholar]
  3. Abdolmajid F, Ghodratollah SS, Hushang R, Mojtaba MB, Ali MM, Abdolghayoum M. 2015. Identification of Leishmania species by kinetoplast DNA-polymerase chain reaction for the first time in Khaf district, Khorasan-e-Razavi province, Iran. Tropical Parasitology, 5(1), 50. [CrossRef] [PubMed] [Google Scholar]
  4. Ahmadizadeh A, Soleimani K, Hosseinpour M, Zakeri A, Nejhad M, Ahmadizadeh A. 2013. Epidemiology of cutaneous leishmaniasis in Hormozgan province (2007–2011). Life Science Journal, 10(3), 1473–1475. [Google Scholar]
  5. Akhlagh A, Salehzadeh A, Zahirnia AH, Davari B. 2019. 10-year trends in epidemiology, diagnosis, and treatment of cutaneous leishmaniasis in Hamadan Province, West of Iran (2007–2016). Frontiers in Public Health, 7, 27. [CrossRef] [PubMed] [Google Scholar]
  6. Akhoundi M, Baghaei A, Depaquit J, Parvizi P. 2013. Molecular characterization of Leishmania infection from naturally infected sand flies caught in a focus of cutaneous leishmaniasis (Eastern Iran). Journal of Arthropod-Borne Diseases, 7(2), 122. [PubMed] [Google Scholar]
  7. Akhoundi M, Hajjaran H, Baghaei A, Mohebali M. 2013. Geographical distribution of Leishmania species of human cutaneous leishmaniasis in Fars Province, Southern Iran. Iranian Journal of Parasitology, 8(1), 85. [PubMed] [Google Scholar]
  8. Akhoundi M, Mohebali M, Asadi M, Mahmodi MR, Amraei K, Mirzaei A. 2013. Molecular characterization of Leishmania spp. in reservoir hosts in endemic foci of zoonotic cutaneous leishmaniasis in Iran. Folia Parasitologica, 60(3), 218. [CrossRef] [PubMed] [Google Scholar]
  9. Alavinia S, Arzamani K, Reihani M, Jafari J. 2009. Some epidemiological aspects of cutaneous leishmaniasis in Northern Khorasan Province, Iran. Iranian Journal of Arthropod-Borne Diseases, 3(2), 50. [PubMed] [Google Scholar]
  10. Amraee K, Rastegar HA, Beiranvand E. 2013. An epidemiological study of cutaneous leishmaniasis in Poledokhtar district, Lorestan province, southwestern of Iran, 2001–2011. Jundishapur Journal of Health Sciences, 5(1), 55–62. [Google Scholar]
  11. Ardehali S, Moattari A, Hatam G, Hosseini S, Sharifi I. 2000. Characterization of Leishmania isolated in Iran: 1. Serotyping with species specific monoclonal antibodies. Acta Tropica, 75(3), 301–307. [CrossRef] [PubMed] [Google Scholar]
  12. Azimi F, Shirian S, Jangjoo S, Ai A, Abbasi T. 2017. Impact of climate variability on the occurrence of cutaneous leishmaniasis in Khuzestan Province, southwestern Iran. Geospatial Health, 12(1), 15–22. [CrossRef] [Google Scholar]
  13. Azizi K, Badzohreh A, Sarkari B, Fakoorziba MR, Kalantari M, Moemenbellah-Fard MD, Ali-Akbarpour M. 2013. Nested polymerase chain reaction and sequence-based detection of Leishmania infection of sand flies in recently emerged endemic focus of zoonotic cutaneous leishmaniasis, southern Iran. Iranian Journal of Medical Sciences, 38(2 Suppl), 156. [PubMed] [Google Scholar]
  14. Azizi K, Kalantari M, Fekri S. 2011. The nested-PCR based detection of cutaneous leishmaniasis vectors in Jask County, Hormozgan, Iran. Iranian Journal of Epidemiology, 7(3), 27–33. [Google Scholar]
  15. Azizi K, Moemenbellah-Fard MD, Kalantari M, Fakoorziba MR. 2012. Molecular detection of Leishmania major kDNA from wild rodents in a new focus of zoonotic cutaneous leishmaniasis in an oriental region of Iran. Vector-Borne and Zoonotic Diseases, 12(10), 844–850. [CrossRef] [PubMed] [Google Scholar]
  16. Azizi K, Parvinjahromi H, Moemenbellah-Fard MD, Sarkari B, Fakoorziba MR. 2016. Faunal distribution and seasonal bio-ecology of naturally infected sand flies in a new endemic zoonotic cutaneous leishmaniasis focus of southern Iran. Journal of Arthropod-Borne Diseases, 10(4), 560. [PubMed] [Google Scholar]
  17. Baghaei A, Jasbi E, Akhoundi M, Mirzaei H, Dehnam O. 2012. Microscopic and molecular detection of Leishmania species among suspected patients of cutaneous leishmaniasis using ITS-r DNA in Fars Province. SSU_Journals, 20(4), 464–473. [Google Scholar]
  18. Baharshahi A, Keikhosravi A, Behravan M. 2017. Identification of Leishmania parasites and sandflies fauna in Zirkouh City, Khorasan-e-Jonoobi Province. Journal of Rafsanjan University of Medical Sciences, 15(10), 903–916. [Google Scholar]
  19. Bamorovat M, Sharifi I, Mohammadi MA, Eybpoosh S, Nasibi S, Aflatoonian MR, Khosravi A. 2018. Leishmania tropica isolates from non-healed and healed patients in Iran: A molecular typing and phylogenetic analysis. Microbial Pathogenesis, 116, 124–129. [CrossRef] [PubMed] [Google Scholar]
  20. Barati H, Lotfi MH, Mozafari G, Dehghan H, Barati M, Tajfirouzeh A. 2016. Epidemiological aspects of cutaneous leishmaniasis in Yazd province within 2004–2013. Journal of Community Health Research, 5(2), 131–139. [Google Scholar]
  21. Bazrafshan M-R, Safari K, Shokrpour N, Delam H. 2020. Epidemiology of cutaneous leishmaniosis in south Fars province, Iran: A retrospective decade long survey. Journal of Health Sciences & Surveillance System, 8(1), 28–33. [Google Scholar]
  22. Behravan M, Hajjaran H, Abadi A, Haghighi A, Rahbarian N, Amini A, Hoobar MH, Khajeh-Farokhi M, Moin-Vaziri V. 2015. Cutaneous leishmaniasis in suspected refereed patients to health centers of Varamin and determination of sand flies species during 2012–2013. Medical Journal of Tabriz University of Medical Sciences, 37(2), 6–11. [Google Scholar]
  23. Behravan M, Moin-Vaziri V, Haghighi A, Rahbarian N, Taghipour N, Abadi A, Hajjaran H. 2017. Molecular identification of Leishmania species in a re-emerged focus of cutaneous leishmaniasis in Varamin district, Iran. Journal of Arthropod-Borne Diseases, 11(1), 124. [PubMed] [Google Scholar]
  24. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL. 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. Journal of Clinical Microbiology, 44(4), 1435–1439. [CrossRef] [PubMed] [Google Scholar]
  25. Bidabadi LS, Nilforoushzadeh M, Akhavan A, Abdoli H, Siadat A, Jaffary F, Hejazi S, Shareghi N, Ghanei M, Arandian M. 2009. Karyosystematic and morphometric characterization of the rodents as reservoir hosts of zoonotic cutaneous leishmaniasis in an endemic focus of Isfahan Province, Iran. Journal of Vector Borne Diseases, 46(1), 52. [PubMed] [Google Scholar]
  26. Bojd AH, Ershadi MMY, Zamani J, Barzekar A, Jafari R, Abazari GP. 2006. Epidemiologic aspect of Cutaneaus Leishmaniasis in Hajiabad, Hormozgan, Iran (2003). Medical Journal of Hormozgan University Medicine Science, 10(1), 63–70. [Google Scholar]
  27. Charrahy Z, Yaghoobi-Ershadi MR, Shirzadi MR, Akhavan AA, Rassi Y, Hosseini SZ, Webb NJ, Haque U, Bozorg Omid F, Hanafi-Bojd AA. 2022. Climate change and its effect on the vulnerability to zoonotic cutaneous leishmaniasis in Iran. Transboundary and Emerging Diseases, 69(3), 1506–1520. [CrossRef] [PubMed] [Google Scholar]
  28. Cherabin M, Sofizadeh A, Palideh A, Yapanggharavi A, Yapang GM. 2012. Epidemiological characteristics of Cautaneous Leishmaniasis in Maraveh tapeh district, Golestan province during 2006–2010. Journal of Zabol University of Medical Sciences and Health Services (Journal of Rostamineh), 4(1), 1–11. [Google Scholar]
  29. Dabirzadeh M, Baghaie M, Hejazi H. 2012. Genetic polymorphism of Leishmania major in two hyper endemic regions of Iran revealed by PPIP-PCR and ITS-RFLP. Archives of Iranian Medicine, 15(3), 151–156. [PubMed] [Google Scholar]
  30. Davami MH, Motazedian MH, Kalantari M, Asgari Q, Mohammadpour I, Sotoodeh-Jahromi A, Solhjoo K, Pourahmad M. 2014. Molecular survey on detection of Leishmania infection in rodent reservoirs in Jahrom District, Southern Iran. Journal of Arthropod-Borne Diseases, 8(2), 139. [PubMed] [Google Scholar]
  31. Doudi M. 2011. The study of genetic polymorphism of Leishmania major and Leishmania tropica by PCR-RFLP technique in two hyper-endemic regions: Isfahan and Bam, Iran. Journal of Arak University of Medical Sciences, 14(4), 20–29. [Google Scholar]
  32. Edrissian GH, Darabian P, Zovein Z, Seyedi-Rashti M, Nadim A. 1981. Application of the indirect fluorescent antibody test in the serodiagnosis of cutaneous and visceral leishmaniasis in Iran. Annals of Tropical Medicine & Parasitology, 75(1), 19–24. [CrossRef] [PubMed] [Google Scholar]
  33. Eslami G, Hajimohammadi B, Jafari AA, Mirzaei F, Gholamrezai M, Anvari H, Khamesipour A. 2014. Molecular identification of Leishmania tropica infections in patients with cutaneous leishmaniasis from an endemic central of Iran. Tropical Biomedicine, 31(4), 592–599. [PubMed] [Google Scholar]
  34. Fakhar M, Motazedian M, Asgari Q, Kalantari M, Hatam G, Akbarpoor M, Gharachahi M. 2010. The efficacy of PCR for early diagnosis and detection of asymptomatic cases of visceral leishmaniasis in human and dog. Journal of Jahrom University of Medical Sciences, 8(2), 1–7. [Google Scholar]
  35. Farahmand M, Nahrevanian H, Shirazi HA, Naeimi S, Farzanehnejad Z. 2011. An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran. Brazilian Journal of Infectious Diseases, 15(1), 17–21. [CrossRef] [Google Scholar]
  36. Feiz-Haddad M-H, Kassiri H, Kasiri N, Panahandeh A, Lotfi M. 2015. Prevalence and epidemiologic profile of acute cutaneous leishmaniasis in an endemic focus, Southwestern Iran. Journal of Acute Disease, 4(4), 292–297. [CrossRef] [Google Scholar]
  37. Fekri S, Hanafi-Bojd AA, Salari Y, Davoodian P, Safari R, Dadvand H, Mohebbi M, Issazadeh H, Kamali Z. 2018. Situational analysis of cutaneous leishmaniasis in an endemic focus of the disease, Southeastern Iran. Journal of Arthropod-Borne Diseases, 12(1), 76. [PubMed] [Google Scholar]
  38. Firooz A, Mortazavi H, Khamesipour A, Ghiasi M, Abedini R, Balighi K, Esmaili N, Nassiri-Kashani M, Eskandari SE, Mohebali M. 2021. Old world cutaneous leishmaniasis in Iran: Clinical variants and treatments. Journal of Dermatological Treatment, 32(7), 673–683. [CrossRef] [PubMed] [Google Scholar]
  39. Fouladi B, Sharifi I, Shahri SMH, Moradgholi H, Sarabandi A, Ebrahimzadeh A, Fazaeli A. 2007. Evaluation of a direct PCR in comparison with routine microscopy and in vitro culture for diagnosis of cutaneous leishmaniasis. Zahedan Journal of Research in Medical Sciences, 9(3), 181–189. [Google Scholar]
  40. Ghaffari D, Hakimi Parizi M, Yaghoobi Ershadi MR, Sharifi I, Akhavan AA. 2014. A survey of reservoir hosts in two foci of cutaneous leishmaniasis in Kerman province, southeast of Iran. Journal of Parasitic Diseases, 38(3), 245–249. [CrossRef] [PubMed] [Google Scholar]
  41. Ghasemloo H, Rasti S, Delavari M, Doroodgar A. 2016. Molecular diagnosis of clinical isolates of cutaneous leishmaniasis using ITS1 and KDNA genes and genetic polymorphism of Leishmania in Kashan, Iran. Pakistan Journal of Biological Sciences, 19(3), 136. [CrossRef] [PubMed] [Google Scholar]
  42. Ghatee MA, Mirhendi H, Marashifard M, Kanannejad Z, Taylor WR, Sharifi I. 2018. Population structure of Leishmania tropica causing anthroponotic cutaneous leishmaniasis in southern Iran by PCR-RFLP of kinetoplastid DNA. BioMed Research International, 2018, 1–11. [CrossRef] [Google Scholar]
  43. Ghias M, Moradpour S, Karimi S. 2019. The comparison of the effects of climatic elements in cutaneous leishmaniosis incidence in two Gilan-e-Gharb and Kermanshah counties, between 2006–2016. Spatial Planning, 9(1), 45–60. [Google Scholar]
  44. Gholamrezaei M, Mohebali M, Hanafi-Bojd AA, Sedaghat MM, Shirzadi MR. 2016. Ecological niche modeling of main reservoir hosts of zoonotic cutaneous leishmaniasis in Iran. Acta Tropica, 160, 44–52. [CrossRef] [PubMed] [Google Scholar]
  45. Haddad MHF, Safaei K, Saki A, Haddad RF. 2016. Epidemiological study of cutaneous leishmaniasis in southwest of Iran during 2001–2011. Asian Pacific Journal of Tropical Disease, 6(6), 432–436. [CrossRef] [Google Scholar]
  46. Hajjaran H, Saberi R, Borjian A, Fakhar M, Hosseini SA, Ghodrati S, Mohebali M. 2021. The geographical distribution of human cutaneous and visceral leishmania species identified by molecular methods in Iran: A systematic review with meta-analysis. Frontiers in Public Health, 9, 835. [CrossRef] [Google Scholar]
  47. Hajjaran H, Vasigheh F, Mohebali M, Rezaei S, Mamishi S, Charedar S. 2011. Direct diagnosis of Leishmania species on serosity materials punctured from cutaneous leishmaniasis patients using PCR-RFLP. Journal of Clinical Laboratory Analysis, 25(1), 20–24. [CrossRef] [PubMed] [Google Scholar]
  48. Hamzavi Y, Khademi N. 2015. Trend of cutaneous leishmaniasis in Kermanshah Province, west of Iran from 1990 to 2012. Iranian Journal of Parasitology, 10(1), 78. [PubMed] [Google Scholar]
  49. Hamzavi Y, Nomanpour B, Karaji AG. 2010. Identification of species of Leishmania isolated from patients with cutaneous leishmaniasis in Kermanshah; using RAPD-PCR technique. Journal of Kermanshah University of Medical Sciences, 14(3), 267–270. [Google Scholar]
  50. Hashemi N, Hashemi M, Eslami G, Bidabadi LS, Hejazi SH. 2012. Detection of Leishmania parasites from cutaneous leishmaniasis patients with negative direct microscopy using NNN and PCR-RFLP. Journal of Isfahan Medical School, 29(169), 1–7.. [Google Scholar]
  51. Hatam G, Ardehali S, Riyad M, Bichichi M, Guessous-Idrissi N, Hejazi S. 2005. Isoenzyme characterization of Iranian Leishmania isolates from cutaneous leishmaniasis. Iranian Journal of Science and Technology Transaction A – Science, 29(A1), 65–70. [Google Scholar]
  52. Holakouie-Naieni K, Mostafavi E, Boloorani AD, Mohebali M, Pakzad R. 2017. Spatial modeling of cutaneous leishmaniasis in Iran from 1983 to 2013. Acta Tropica, 166, 67–73. [CrossRef] [PubMed] [Google Scholar]
  53. Izadi S, Mirhendi H, Jalalizand N, Khodadadi H, Mohebali M, Nekoeian S, Jamshidi A, Ghatee MA. 2016. Molecular epidemiological survey of cutaneous leishmaniasis in two highly endemic metropolises of Iran, application of FTA cards for DNA extraction from Giemsa-stained slides. Jundishapur Journal of Microbiology, 9(2), 1–7. [CrossRef] [Google Scholar]
  54. Jafari R, Najafzadeh N, Sedaghat M, Parvizi P. 2013. Molecular characterization of sandflies and Leishmania detection in main vector of zoonotic cutaneous leishmaniasis in Abarkouh district of Yazd province, Iran. Asian Pacific Journal of Tropical Medicine, 6(10), 792–797. [CrossRef] [PubMed] [Google Scholar]
  55. Jafarnejad A, Jamshidi F, Deghan A. 2017. Evaluation of cutaneous leishmaniasis in the city of Lamerd in 2004–2014. Medical Journal of Mashhad University of Medical Sciences, 60(1), 376–382. [Google Scholar]
  56. Jahromi S, Madani A. 2018. Epidemiology of cutaneous leishmaniasis, West South of Iran. Journal of Research in Medical and Dental Science, 6(2), 378. [Google Scholar]
  57. Javadian E, Dehestani M, Nadim A, Rassi Y, Tahvildar-Bidruni G, Seyedi-Rashti M, Shadmehr A. 1998. Confirmation of Tatera indica (Rodentia: Gerbilldae) as the main reservoir host of zoonotic cutaneous leishmaniasis in the west of Iran. Iranian Journal of Public Health, 27(1–2), 55–60. [Google Scholar]
  58. Kalantari M, Motazedian MH, Asgari Q, Mohammadpour I, Soltani A, Azizi K. 2018. Co-detection and isolation of Leishmania and Crithidia among naturally infected Tatera indica (Rodentia: Muridae) in Fars province, southern Iran. Asian Pacific Journal of Tropical Biomedicine, 8(5), 279. [CrossRef] [Google Scholar]
  59. Karami M, Doudi M, Setorki M. 2013. Assessing epidemiology of cutaneous leishmaniasis in Isfahan, Iran. Journal of Vector Borne Diseases, 50(1), 30. [PubMed] [Google Scholar]
  60. Karimi A, Hanafi-Bojd AA, Yaghoobi-Ershadi MR, Akhavan AA, Ghezelbash Z. 2014. Spatial and temporal distributions of phlebotomine sand flies (Diptera: Psychodidae), vectors of leishmaniasis, in Iran. Acta Tropica, 132, 131–139. [CrossRef] [PubMed] [Google Scholar]
  61. Kassiri H, Farhadinejad R, Lotfi M. 2018. Survey on epidemiological status and incidence rate of cutaneous leishmaniasis in Abadan county, Khuzestan province, Southwestern Iran. Entomology and Applied Science Letters, 5(2), 62–69. [Google Scholar]
  62. Kassiri H, Javadian E, Abdigoudarzi M. 2011. Natural Leishmania infection in Meriones hurrianae and Tatera indica (Rodentia: Cricetidae: Gerbillinae) in Sistan-Baluchistan Province, South-Eastern of Iran. Advanced Studies in Biology, 3(6), 247–256. [Google Scholar]
  63. Kassiri H, Lotfi M, Farajifard P, Kassiri E. 2014. Laboratory diagnosis, clinical manifestations, epidemiological situation and public health importance of cutaneous leishmaniasis in Shushtar County, Southwestern Iran. Journal of Acute Disease, 3(2), 93–98. [CrossRef] [Google Scholar]
  64. Kassiri H, Sharifinia N, Jalilian M, Shemshad K. 2012. Epidemiological aspects of cutaneous leishmaniasis in Ilam province, west of Iran (2000–2007). Asian Pacific Journal of Tropical Disease, 2, S382–S386. [CrossRef] [Google Scholar]
  65. Kassiri H, Shemshad K, Kassiri A, Shojaee S, Sharifinia N, Shemshad M. 2012. Clinical laboratory and epidemiological research on cutaneous leishmaniasis in the south west of Iran. Archives of Clinical Infectious Diseases, 7(4), 128–131. [CrossRef] [Google Scholar]
  66. Kassiri H, Shemshad K, Lotfi M, Shemshad M. 2013. Relationship trend analysis of cutaneous leishmaniasis prevalence and climatological variables in Shush county, south-west of Iran (2003–2007). Academic Journal of Entomology, 6(2), 79–84. [Google Scholar]
  67. Kavarizadeh F, Khademvatan S, Vazirianzadeh B, Feizhaddad MH, Zarean M. 2017. Molecular characterization of Leishmania parasites isolated from sandflies species of a zoonotic cutaneous leishmaniasis in Musiyan south west Iran. Journal of Parasitic Diseases, 41(1), 274–281. [CrossRef] [PubMed] [Google Scholar]
  68. Kavarizadeh F, Vazirianzadeh B, Rassi Y, Jalali Glusang A, Abbas Moravvej S. 2013. A faunistic study of sand flies of Musian District, Southwestern of Iran. Pakistan Journal of Zoology, 45(2), 549–554. [Google Scholar]
  69. Kermanjani A, Akhlaghi L, Oormazdi H, Hadighi R. 2017. Isolation and identification of cutaneous leishmaniasis species by PCR–RFLP in Ilam province, the west of Iran. Journal of Parasitic Diseases, 41(1), 175–179. [CrossRef] [PubMed] [Google Scholar]
  70. Khademvatan S, Salmanzadeh S, Foroutan-Rad M, Bigdeli S, Hedayati-Rad F, Saki J, Heydari-Gorji E. 2017. Spatial distribution and epidemiological features of cutaneous leishmaniasis in southwest of Iran. Alexandria Journal of Medicine, 53(1), 93–98. [CrossRef] [Google Scholar]
  71. Khajedaluee M, Yazdanpanah MJ, SeyedNozadi S, Fata A, Juya MR, Masoudi MH, Najaf Najafi M. 2014. Epidemiology of cutaneous leishmaniasis in population covered by Mashhad University of Medical Sciences in 2011. Medical Journal of Mashhad University of Medical Sciences, 57(4), 647–654. [Google Scholar]
  72. Khazaei S, Hafshejani AM, Saatchi M, Salehiniya H, Nematollahi S. 2015. Epidemiological aspects of cutaneous leishmaniasis in Iran. Archives of Clinical Infectious Diseases, 10(3), 1–5. [CrossRef] [Google Scholar]
  73. Kheirandish F, Sharafi AC, Kazemi B, Bandehpour M, Javad Tarahi M, Khamesipour A. 2013. First molecular identification of Leishmania species in a new endemic area of cutaneous leishmaniasis in Lorestan, Iran. Asian Pacific Journal of Tropical Medicine, 6(9), 713–717. [CrossRef] [PubMed] [Google Scholar]
  74. Khosravani M, Moemenbellah-Fard MD, Sharafi M, Rafat-Panah A. 2016. Epidemiologic profile of oriental sore caused by Leishmania parasites in a new endemic focus of cutaneous leishmaniasis, southern Iran. Journal of Parasitic Diseases, 40(3), 1077–1081. [CrossRef] [PubMed] [Google Scholar]
  75. Khosravani M, Nasiri Z, Keshavarz D, Rafat-Panah A. 2016. Epidemiological trend of cutaneous leishmaniasis in two endemic focus of disease, south of Iran. Journal of Parasitic Diseases, 40(4), 1609–1613. [CrossRef] [PubMed] [Google Scholar]
  76. Khosravani Poor H, Ali Akbar Poor M, Zare Askari A, Danesh M, Keshavarz A, Palizian B. 2019. Lifetime prevalence of cutaneous leishmaniasis scar in the city of Kherameh, Fars province, Iran (2015). Journal of Occupational Health and Epidemiology, 8(1), 12–20. [CrossRef] [Google Scholar]
  77. Khosravi A, Sharifi I, Dortaj E, Afshar AA, Mostafavi M. 2013. The present status of cutaneous leishmaniasis in a recently emerged focus in south-west of Kerman Province, Iran. Iranian Journal of Public Health, 42(2), 182. [PubMed] [Google Scholar]
  78. Mahmoodi MR, Mohajery M, Afshari JT, Shakeri MT, Yazdan Panah MJ, Berenji F, Fata A. 2010. Molecular identification of Leishmania species causing cutaneous leishmaniasis in Mashhad, Iran. Jundishapur Journal of Microbiology, 3(4), 195–200. [Google Scholar]
  79. Mahmoudzadeh-Niknam H, Ajdary S, Riazi-Rad F, Mirzadegan E, Rezaeian A, Khaze V, Djadid ND, Alimohammadian MH. 2012. Molecular epidemiology of cutaneous leishmaniasis and heterogeneity of Leishmania major strains in Iran. Tropical Medicine & International Health, 17(11), 1335–1344. [CrossRef] [PubMed] [Google Scholar]
  80. Mahnaz T, Katrin K, Amer A-J, Isabel M, Gabriele S, Safar F, Hossein AM. 2006. Leishmania major: Genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer. Acta Tropica, 98(1), 52–58. [CrossRef] [PubMed] [Google Scholar]
  81. Marfurt J, Nasereddin A, Niederwieser I, Jaffe CL, Beck H-P, Felger I. 2003. Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. Journal of Clinical Microbiology, 41(7), 3147–3153. [CrossRef] [PubMed] [Google Scholar]
  82. Masoumeh A, Kourosh A, Mohsen K, Hossein MM, Qasem A, Djaefar M-FM, Esmaeil NM, Tahereh D. 2014. Laboratory based diagnosis of leishmaniasis in rodents as the reservoir hosts in southern Iran, 2012. Asian Pacific Journal of Tropical Biomedicine, 4, S575–S580. [CrossRef] [Google Scholar]
  83. Mehrabani D, Motazedian M, Hatam G, Asgari Q, Owji S, Oryan A. 2011. Leishmania major in Tatera indica in Fasa, southern Iran: Microscopy, culture, isoenzyme, PCR and morphologic study. Asian Journal of Animal and Veterinary Advances, 6(3), 255–264. [CrossRef] [Google Scholar]
  84. Mehrabani D, Motazedian M, Oryan A, Asgari Q, Hatam G, Karamian M. 2007. A search for the rodent hosts of Leishmania major in the Larestan region of southern Iran: Demonstration of the parasite in Tatera indica and Gerbillus sp., by microscopy, culture and PCR. Annals of Tropical Medicine & Parasitology, 101(4), 315–322. [CrossRef] [PubMed] [Google Scholar]
  85. Mirzaei A, Rouhani S, Kazerooni P, Farahmand M, Parvizi P. 2013. Molecular detection and conventional identification of Leishmania species in reservoir hosts of zoonotic cutaneous leishmaniasis in Fars province, South of Iran. Iranian Journal of Parasitology, 8(2), 280. [PubMed] [Google Scholar]
  86. Mirzaei A, Rouhani S, Taherkhani H, Farahmand M, Kazemi B, Hedayati M, Baghaei A, Davari B, Parvizi P. 2011. Isolation and detection of Leishmania species among naturally infected Rhombomis opimus, a reservoir host of zoonotic cutaneous leishmaniasis in Turkemen Sahara, North East of Iran. Experimental Parasitology, 129(4), 375–380. [CrossRef] [PubMed] [Google Scholar]
  87. Moein D, Masoud D, Saeed M, Abbas D. 2018. Epidemiological aspects of cutaneous leishmaniasis during 2009–2016 in Kashan city, central Iran. Korean Journal of Parasitology, 56(1), 21. [CrossRef] [PubMed] [Google Scholar]
  88. Moemenbellah-Fard M, Kalantari M, Rassi Y, Javadian E. 2003. The PCR-based detection of Leishmania major infections in Meriones libycus (Rodentia: Muridae) from southern Iran. Annals of Tropical Medicine and Parasitology, 97(8), 811–816. [PubMed] [Google Scholar]
  89. Mohammadi Azni S, Nokandeh Z, Khorsandi A, Sanei Dehkordi AR. 2010. Epidemiology of cutaneous leishmaniasis in Damghan district. Journal of Military Medicine, 12(3), 131–135. [Google Scholar]
  90. Mohammadi S, Vazirianzadeh B, Fotouhi-Ardakani R, Novin EA, Amirkhani A, Samii J, Rastaghi ARE, Parvizi P. 2017. Tatera indica (Rodentia: Muridae) as the prior concern and the main reservoir host of zoonotic cutaneous leishmaniasis on the border of Iran and Iraq. Jundishapur Journal of Microbiology, 10(1), 1–8. [Google Scholar]
  91. Mokhtari M, Miri M, Nikoonahad A, Jalilian A, Naserifar R, Ghaffari HR, Kazembeigi F. 2016. Cutaneous leishmaniasis prevalence and morbidity based on environmental factors in Ilam, Iran: Spatial analysis and land use regression models. Acta Tropica, 163, 90–97. [CrossRef] [PubMed] [Google Scholar]
  92. Mollalo A, Alimohammadi A, Shahrisvand M, Shirzadi MR, Malek MR. 2014. Spatial and statistical analyses of the relations between vegetation cover and incidence of cutaneous leishmaniasis in an endemic province, northeast of Iran. Asian Pacific Journal of Tropical Disease, 4(3), 176–180. [CrossRef] [Google Scholar]
  93. Mollalo A, Alimohammadi A, Shirzadi MR, Malek MR. 2015. Geographic information system-based analysis of the spatial and spatio-temporal distribution of zoonotic cutaneous leishmaniasis in Golestan Province, north–east of Iran. Zoonoses and Public Health, 62(1), 18–28. [PubMed] [Google Scholar]
  94. Moosazadeh M, Afshari M, Parsaee M, Nezammahalleh A, Abedi G, Fakhar M, Mahdavi SA, Shojaei J, Enayati AA. 2019. Clinico-epidemiological features of cutaneous leishmaniasis in Mazandaran Province, northern Iran. Clinical Epidemiology and Global Health, 7(3), 378–381. [CrossRef] [Google Scholar]
  95. Moradi M, Rassi Y, Abai MR, Zahraei Ramazani A, Mohebali M, Rafizadeh S. 2018. Some epidemiological aspects of cutaneous leishmaniasis with emphasis on vectors and reservoirs of disease in the borderline of Iran and Iraq. Journal of Parasitic Diseases, 42(2), 243–251. [CrossRef] [PubMed] [Google Scholar]
  96. Motazedian MH, Parhizkari M, Mehrabani D, Hatam G, Asgari Q. 2010. First detection of Leishmania major in Rattus norvegicus from Fars province, southern Iran. Vector-Borne and Zoonotic Diseases, 10(10), 969–975. [CrossRef] [PubMed] [Google Scholar]
  97. Nassif PW, De Mello TFP, Navasconi TR, Mota CA, Demarchi IG, Aristides SMA, Lonardoni MVC, Teixeira JJV, Silveira TGV. 2017. Safety and efficacy of current alternatives in the topical treatment of cutaneous leishmaniasis: A systematic review. Parasitology, 144(8), 995–1004. [CrossRef] [PubMed] [Google Scholar]
  98. Nikonahad A, Khorshidi A, Ghaffari HR, Aval HE, Miri M, Amarloei A, Nourmoradi H, Mohammadi A. 2017. A time series analysis of environmental and metrological factors impact on cutaneous leishmaniasis incidence in an endemic area of Dehloran, Iran. Environmental Science and Pollution Research, 24(16), 14117–14123. [CrossRef] [PubMed] [Google Scholar]
  99. Nikouee F, Soltanian M, Babaee F, Motamed-Jahromi M. 2017. Cutaneous leishmaniasis: An epidemiological survey in Iran during 2013–2015. Journal of Nursing and Midwifery Sciences, 4(1), 58–62. [Google Scholar]
  100. Nozari M, Ansari Shiri M, Samaei MR, Shirdarreh MR, Gholamnejad A, Rezaeian S. 2017. The epidemiological study of cutaneous leishmaniasis in patients referred to skin lesions in Dashtestan District, Bushehr Province, Iran in 2013–2014. Journal of Environmental Health and Sustainable Development, 2(4), 388–398. [Google Scholar]
  101. Oshaghi M, Yaghobi-Ershadi M, Abbassi M, Parvizi P, Akhavan A, Foroshani AR, Zahraei A, Rassi Y, Mohtarami F. 2008. Detection of Leishmania major in naturally infected sand flies using semi nested-PCR. Iranian Journal of Public Health, 37(4), 59–64. [Google Scholar]
  102. Ostad M, Shirian S, Pishro F, Abbasi T, Ai A, Azimi F. 2016. Control of cutaneous leishmaniasis using geographic information systems from 2010 to 2014 in Khuzestan Province, Iran. PLoS ONE, 11(7), e0159546. [CrossRef] [PubMed] [Google Scholar]
  103. Pagheh AS, Fakhar M, Mesgarian F, Rahimi-Esboei B, Badiee F. 2013. Incidence trend of rural cutaneous leishmaniasis in Gonbad-e-Qabus city, (Golestan, Iran) during 2009–2012. Journal of Mazandaran University of Medical Sciences, 23(104), 27–33. [Google Scholar]
  104. Parhizkari M, Motazedian M, Asqari Q, Mehrabani D. 2011. The PCR-based detection of Leishmania major in Mus musculus and other rodents caught in southern Iran: A guide to sample selection. Annals of Tropical Medicine & Parasitology, 105(4), 319–323. [CrossRef] [PubMed] [Google Scholar]
  105. Parvizi P, Alaeenovin E, Kazerooni P, Ready P. 2013. Low diversity of Leishmania parasites in sandflies and the absence of the great gerbil in foci of zoonotic cutaneous leishmaniasis in Fars province, southern Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene, 107(6), 356–362. [CrossRef] [PubMed] [Google Scholar]
  106. Parvizi P, Hedayati M. 2010. Leishmania infections in rodents, reservoir hosts of zoonotic cutaneous leismaniasis in turkemen sahara, gonbad, golastan province. Journal of Guilan University of Medical Sciences, 18(72), 30–38. [Google Scholar]
  107. Pashapour S, Heshmati M, Mousavi Z, Esmaeili S. 2020. The cytotoxicity of the chloroform and petroleum ether fractional extracts of Galium verum L. in HepG2 and HT29 cell lines. Journal of Kermanshah University of Medical Sciences, 24(2), 1–5. [CrossRef] [Google Scholar]
  108. Pourmohammadi B, Mohammadi-Azni S, Kalantari M. 2017. Natural infection of Nesokia indica with Leishmania major and Leishmania infantum parasites in Damghan city, Northern Iran. Acta Tropica, 170, 134–139. [CrossRef] [PubMed] [Google Scholar]
  109. Pourmohammadi B, Motazedian M, Hatam G, Kalantari M, Habibi P, Sarkari B. 2010. Comparison of three methods for diagnosis of cutaneous leishmaniasis. Iranian Journal of Parasitology, 5(4), 1. [PubMed] [Google Scholar]
  110. Rajabzadeh R, Arzamani K, Shoraka HR, Riyhani H. 2015. Epidemiological survey and geographical distribution of cutaneous leishmaniasis in North Khorasan province, 2006–2013. International Journal of Epidemiologic Research, 2(4), 197–203. [Google Scholar]
  111. Ramezani Y, Mousavi SGA, Bahrami A, Fereydooni M, Parsa N, Kazemi B. 2011. Epidemiological study of cutaneous leishmaniasis in Aran and Bidgol from April to September 2009. KAUMS Journal (FEYZ), 15(3), 254–258. [Google Scholar]
  112. Ramezankhani R, Hosseini A, Sajjadi N, Khoshabi M, Ramezankhani A. 2017. Environmental risk factors for the incidence of cutaneous leishmaniasis in an endemic area of Iran: A GIS-based approach. Spatial and Spatio-Temporal Epidemiology, 21, 57–66. [CrossRef] [PubMed] [Google Scholar]
  113. Rasi Y, Javadian E, Jalali M, Motazedian M, Vatndoost H. 2004. Investigation on zoonotic cutaneous leishmaniasis, Southern Iran. Iranian Journal of Public Health, 33(1), 31–35. [Google Scholar]
  114. Rasi Y, Mohammadi Azni S, Oshaghi MA, Yaghoubi Ershadi M, Mohebali M, Abaei M, Mohtarami F, Nokandeh Z, Rafizadeh S. 2012. Study on sand flies as a vector (s) of cutaneous leishmaniasis by nested PCR in rural areas of Damghan District, Semnan Province. Avicenna Journal of Clinical Medicine, 18(4), 47–52. [Google Scholar]
  115. Rassi Y, Gassemi M, Javadian E, Rafizadeh S, Motazedian H, Vatandoost H. 2007. Vectors and reservoirs of cutaneous leishmaniasis in Marvdasht district, southern Islamic Republic of Iran. EMHJ-Eastern Mediterranean Health Journal, 13(3), 686–693. [Google Scholar]
  116. Rassi Y, Jalali M, Javadian E, Moatazedian M. 2001. Confirmation of Meriones libycus (Rodentia; Gerbillidae) as the main reservoir host of zoonotic cutaneous leishmaniasis in Arsanjan, Fars province, South of Iran (1999–2000). Iranian Journal of Public Health, 30(3–4), 143–144. [Google Scholar]
  117. Rassi Y, Javadian E, Amin M, Rafizadeh S, Vatandoost H, Motazedian H. 2006. Meriones libycus is the main reservoir of zoonotic cutaneous leishmaniasis in south Islamic Republic of Iran. EMHJ – Eastern Mediterranean Health Journal, 12(3–4), 474–477. [Google Scholar]
  118. Rassi Y, Oshaghi MA, Yaghoobi Ershdi MR, Mohebali M, Abai MR, Mohtarami F, Nokandeh Z, Rafizadeh S, Khojami GhM. 2011. Determination of parasite species of cutaneous leishmaniasis using nested PCR in Damghan-Iran, during 2008. Journal of Gorgan University of Medical Sciences, 13(1), 59–65. [Google Scholar]
  119. Rassi Y, Oshaghi MA, Azani SM, Abaie MR, Rafizadeh S, Mohebai M, Mohtarami F, Mk Zeinali. 2011. Molecular detection of Leishmania infection due to Leishmania major and Leishmania turanica in the vectors and reservoir host in Iran. Vector-Borne and Zoonotic Diseases, 11(2), 145–150. [CrossRef] [PubMed] [Google Scholar]
  120. Rassi Y, Saghafipour A, Abai MR, Oshaghi MA, Mohebali M, Mostafavi R. 2013. Determination of Leishmania parasite species of cutaneous leishmaniasis using PCR method in Central County, Qom Province. Zahedan Journal of Research in Medical Sciences, 15(12), 13–16. [Google Scholar]
  121. Reithinger R, Dujardin J-C. 2007. Molecular diagnosis of leishmaniasis: Current status and future applications. Journal of Clinical Microbiology, 45(1), 21–25. [CrossRef] [PubMed] [Google Scholar]
  122. Rezaee N, Shamsi M, Damani E, Sekandarpour S, Ranjbar N, Mohamdi A, Faizei F. 2019. Prevalence and epidemiologic profile of acute cutaneous leishmaniasis in an endemic focus in Southeast Iran. Hormozgan Medical Journal, 22(4), 1–6. [Google Scholar]
  123. Rezai A, Moghaddas E, Bagherpor MR, Naseri A, Shamsian SA. 2017. Identification of Leishmania species for cutaneous leishmaniasis in Gonabad, Bardaskan and Kashmar, Central Khorasan, 2015. Jundishapur Journal of Microbiology, 10(4), 1–5. [Google Scholar]
  124. Rezvan H, Hamoon Navard S. 2017. An overview on Leishmania diagnosis. Journal of Medical Microbiology and Infectious Diseases, 5(1), 1–11. [CrossRef] [Google Scholar]
  125. Rostami MN, Saghafipour A, Vesali E. 2013. A newly emerged cutaneous leishmaniasis focus in central Iran. International Journal of Infectious Diseases, 17(12), e1198–e1206. [CrossRef] [Google Scholar]
  126. Rostamian M, Bashiri H, Yousefinejad V, Bozorgomid A, Sohrabi N, Raeghi S, Khodayari MT, Ghadiri K, Rezaeian S. 2021. Prevalence of human visceral leishmaniasis in Iran: A systematic review and meta-analysis. Comparative Immunology, Microbiology and Infectious Diseases, 75, 101604. [CrossRef] [PubMed] [Google Scholar]
  127. Saadabadi F, Mohajery M, Poostchi E, Shamsian SAA. 2013. Identification of Leishmania species causing cutaneous leishmaniasis using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve. Iran. Reports of Biochemistry & Molecular Biology, 1(2), 69. [Google Scholar]
  128. Saberi S, Hejazi SH, Jafari R, Bahadoran M, Akbari M, Soleymanifard S, Arjmand R, Alidadi P, Aminian K, Arandian M-H. 2013. The cutaneous leishmaniasis reservoirs in northern Baraan region of Isfahan, Iran. Journal of Isfahan Medical School, 31(253), 1497–1507. [Google Scholar]
  129. Sabzevari S, Teshnizi SH, Shokri A, Bahrami F, Kouhestani F. 2021. Cutaneous leishmaniasis in Iran: A systematic review and meta-analysis. Microbial pathogenesis, 152, 104721. [CrossRef] [PubMed] [Google Scholar]
  130. Sakhaei S, Darrudi R, Motaarefi H, Sadagheyani HE. 2019. Epidemiological study of cutaneous leishmaniasis in Neyshabur county, East of Iran (2011–2017). Open Access Macedonian Journal of Medical Sciences, 7(21), 3710. [CrossRef] [PubMed] [Google Scholar]
  131. Saki J, Khademvatan S. 2011. A molecular study on cutaneous leishmaniasis lesions in Khuzestan province (South west of Iran). Jundishapur Journal of Microbiology, 4(4), 283–288. [Google Scholar]
  132. Sargolzaei N, Tabatabei SM, Hasanzehi A, Kiani M, Jahangiri M. 2015. Evaluation of cutaneous leishmaniasis in central and southern parts of Sistan and Baluchistan Province during April 2008 to March 2014. International Journal of Infection, 2(4), 1–8. [CrossRef] [Google Scholar]
  133. Shahidi-Hakak F, Aivazi AA, Mokhtari F, Jalilian A, Khosravani M, Rafatpanah A. 2020. Typical features of cutaneous leishmaniasis in the Ilam province, Iran. Journal of Parasitic Diseases, 44(4), 748–753. [CrossRef] [PubMed] [Google Scholar]
  134. Shamsian S, Rezaei A, Akbarzadeh A, Hosseini Farash B. 2015. Molecular Identification of Leishmania tropica in an endemic border city for zoonotic cutaneous leishmaniasis (ZCL) in northeastern Iran. Journal of Microbiology & Experimentation, 2(4), 53–58. [Google Scholar]
  135. Sharafi M, Pezeshki B, Reisi A, Kalantari M, Naghizadeh MM, Dast Manesh S. 2013. Detection of cutaneous leishmaniasis by PCR in Fasa district in 2012. Journal of Fasa University of Medical Sciences, 3(3), 266–270. [Google Scholar]
  136. Sharifi I, Aflatoonian MR, Fekri AR, Parizi MH, Afshar AA, Khosravi A, Sharifi F, Aflatoonian B, Khamesipour A, Dowlati Y. 2015. A comprehensive review of cutaneous leishmaniasis in Kerman province, southeastern Iran-narrative review article. Iranian Journal of Public Health, 44(3), 299. [PubMed] [Google Scholar]
  137. Shiee MR, Mohebali M, Doroodgar A, Teimouri A, Afzali H, Shirzadi MR. 2012. A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran. Asian Pacific Journal of Tropical Disease, 2(6), 421–425. [CrossRef] [Google Scholar]
  138. Shirani-Bidabadi N, Nasrabadi T, Faryadi S, Larijani A, Roodposhti MS. 2019. Evaluating the spatial distribution and the intensity of urban heat island using remote sensing, case study of Isfahan city in Iran. Sustainable Cities and Society, 45, 686–692. [CrossRef] [Google Scholar]
  139. Shirzadi MR, Javanbakht M, Vatandoost H, Jesri N, Saghafipour A, Fouladi-Fard R, Omidi-Oskouei A. 2020. Impact of environmental and climate factors on spatial distribution of cutaneous leishmaniasis in northeastern Iran: Utilizing remote sensing. Journal of Arthropod-Borne Diseases, 14(1), 56. [PubMed] [Google Scholar]
  140. Soleimanpoor H, Dabirzadeh M, Fooladi B. 2016. Identification of species causing cutaneous leishmaniasis by PCR in Chahbahar, Iran. Medical Laboratory Journal, 10(2), 46–51. [Google Scholar]
  141. Soltani S, Foroutan M, Hezarian M, Afshari H, Kahvaz MS. 2019. Cutaneous leishmaniasis: An epidemiological study in southwest of Iran. Journal of Parasitic Diseases, 43(2), 190–197. [CrossRef] [PubMed] [Google Scholar]
  142. Steinfeld B, Scott J, Vilander G, Marx L, Quirk M, Lindberg J, Koerner K. 2015. The role of lean process improvement in implementation of evidence-based practices in behavioral health care. Journal of Behavioral Health Services & Research, 42(4), 504–518. [CrossRef] [PubMed] [Google Scholar]
  143. Talari S, Rezvan Talaei R, Shajari G. 2006. A clinical and epidemiology survey of cutaneous leishmaniasis in childhood. Journal of Medical Sciences, 6(4), 693–697. [CrossRef] [Google Scholar]
  144. Tolouei S, Hejazi SH, Ghaedi K, Hasheminia SJ. 2014. Identification of Leishmania isolates from healing and nonhealing cutaneous leishmaniasis patients using internal transcribed spacer region PCR. Jundishapur Journal of Microbiology, 7(4), 1–4. [CrossRef] [Google Scholar]
  145. Torres-Guerrero E, Quintanilla-Cedillo MR, Ruiz-Esmenjaud J, Arenas R. 2017. Leishmaniasis: A review. F1000Research, 6, 750–765. [CrossRef] [PubMed] [Google Scholar]
  146. Vaeznia H, Dalimi A, Sadraei J, Pirstani M. 2009. Determination of Leishmania species causing cutaneous leishmaniasis in Mashhad by PCR-RFLP method. Archives of Razi Institute, 64(1), 39–44. [Google Scholar]
  147. Von Elm E, Altman DG, Egger M, Pocock SJ, Gøtzsche PC, Vandenbroucke JP, Initiative S. 2014. The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement: Guidelines for reporting observational studies. International Journal of Surgery, 12(12), 1495–1499. [CrossRef] [Google Scholar]
  148. Yaghoobi-Ershadi M. 2012. Phlebotomine sand flies (Diptera: Psychodidae) in Iran and their role on Leishmania transmission. Journal of Arthropod-Borne Diseases, 6(1), 1. [PubMed] [Google Scholar]
  149. Yaghoobi-Ershadi M, Akhavan A, Mohebali M. 1996. Meriones Iibycus and Rhombomys opimus (Rodentia: Gerbillidae) are the main reservoir hosts in a new focus of zoonotic cutaneous leishmaniasis in Iran. Transactions of the Royal Society of Tropical Medicine and Hygiene, 90(5), 503–504. [CrossRef] [PubMed] [Google Scholar]
  150. Yaghoobi-Ershadi M, Marvi-Moghadam N, Jafari R, Akhavan A, Solimani H, Zahrai-Ramazani A, Arandian M, Dehghan-Dehnavi A. 2015. Some epidemiological aspects of cutaneous leishmaniasis in a new focus, central Iran. Dermatology Research and Practice, 2015, 1–5. [CrossRef] [Google Scholar]
  151. Yaghoobi Ershadi M, Akhavan A, Zahraei Ramazani A, Abai M, Ebrahimi B, Vafaei Nezhad R, Hanafi Bojd A, Jafari R. 2003. Epidemiological study in a new focus of cutaneous leishmaniasis in the Islamic Republic of Iran. EMHJ-Eastern Mediterranean Health Journal, 9(4), 816–826. [CrossRef] [Google Scholar]
  152. Yavar R, Abedin S, Reza AM, Ali OM, Sina R, Mehdi M, Reza Y-EM, Fatemeh M, Babak F. 2011. Phlebotomus papatasi and Meriones libycus as the vector and reservoir host of cutaneous leishmaniasis in Qomrood District, Qom Province, Central Iran. Asian Pacific Journal of Tropical Medicine, 4(2), 97–100. [CrossRef] [PubMed] [Google Scholar]
  153. Yazdanpanah HA, Rostamianpur M. 2013. Analysis of spatial distribution of leishmaniasis and its relationship with climatic parameters (case study: Ilam Province). Bulletin of Environment, Pharmacology and Life Sciences, 2(12), 80–86. [Google Scholar]
  154. Zahraei-Ramazani A, Sedaghat M. 2016. Checklist and geographical distribution of phlebotomine sand flies (Diptera: Psychodidae) vectors of leishmaniasis in Esfahan province, Iran. IAU Entomological Research Journal, 7(4), 345–358. [Google Scholar]
  155. Zare-Zadeh A, Motalleb G, Mirahmadi H, Salimi Khorashad A. 2019. ITS-rDNA and molecular typing of Leishmania spp. in suspected patients with cutaneous leishmaniasis in Sistan and Balochestan province, Iran. Cellular and Molecular Researches (Iranian Journal of Biology), 32(1), 125–135. [Google Scholar]

Cite this article as: Nasiri Z, Kalantari M, Mohammadi J, Daliri S, Mehrabani D & Azizi K. 2022. Cutaneous leishmaniasis in Iran: A review of epidemiological aspects, with emphasis on molecular findings. Parasite 29, 47.

All Tables

Table 1

Reservoirs of cutaneous leishmaniasis in Iran.

In the text
Table 2

Vectors of anthroponotic cutaneous leishmaniasis (ACL) and zoonotic cutaneous leishmaniasis (ZCL) Iran.

In the text
Table 3

Different Leishmania identification methods in human cases carried out in Iran.

In the text

All Figures

thumbnail Fig. 1

The PRISMA flow diagram.
In the text
thumbnail Fig. 2

Distribution of cutaneous leishmaniasis reservoirs in Iran; 1: Meriones libycus, 2: Rhombomys opimus, 3: Gerbillus nanus, 4: Tatera indica, 5: Meriones persicus, 6: Nesokia indica, 7: Rattus norvegicus, 8: Meriones hurrianae, 9: Mus musculus, 10: Rattus rattus.
In the text
thumbnail Fig. 3

Distribution of anthroponotic cutaneous leishmaniasis in vectors in Iran; A: Phlebotomus papatasi, B: Phlebotomus Salehi, C: Phlebotomus sergenti, D: Phlebotomus caucasicus, E: Phlebotomus mongolensis, F: Phlebotomus anderjevi, I: Phlebotomus ansarii, J: Phlebotomus alexandri, K: Phlebotomus perfiliewi, L: Phlebotomus jacusieli, M: Sergentomyia dentata, N: Sergentomyia sintoni, O: Sergentomyia clydei.
In the text
thumbnail Fig. 4

Distribution of zoonotic cutaneous leishmaniasis in vectors in Iran; 1: Phlebotomus papatasi, 2: Phlebotomus Salehi, 3: Phlebotomus sergenti, 4: Phlebotomus caucasicus, 5: Phlebotomus mongolensis, 6: Phlebotomus ansarii, 7: Phlebotomus alexandri, 8: Sergentomyia dentata, 9: Phlebotomus anderjevi, 10: Phlebotomus tobbi, 11: Sergentomyia theodori, 12: Sergentomyia clydei, 13: Sergentomyia Baghdadis, 14: Sergentomyia squamipleuris, 15: Phlebotomus bergeroti, 16: Sergentomyia sintoni, 17: Sergentomyia tiberiadis, 18: Phlebotomus jacusieli.
In the text

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