Molecular genetic diversity and bioinformatic analysis of Leucocytozoon sabrazesi based on the mitochondrial genes cytb, coxI and coxIII and co-infection of Plasmodium spp.

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Table 2

Comparison of nucleotide sequence analyses of three mitochondrial and concatenated genes of Leucocytozoon spp. and Plasmodium spp. as detected in chicken samples in Thailand and other countries.

Comparison	cytb		coxI		coxIII		Concatenated gene
Comparison	Leucocytozoon spp.	Plasmodium spp.	Leucocytozoon spp.	Plasmodium spp.	Leucocytozoon spp.	Plasmodium spp.	Leucocytozoon spp.	Plasmodium spp.
No. of polymorphic sites	65	46	228	104	93	73	149	123
No. of mutations	78	52	304	122	102	84	162	136
Nucleotide difference (k)	13.153	18.857	64.332	38	17.146	28.927	26.83	48.139
Nucleotide diversity (Pi)	0.06354	0.0911	0.162286	0.0962	0.07056	0.11904	0.06002	0.1079
No. of haplotypes (h)	12	8	19	13	14	10	13	8
	cytb		coxI		coxIII		Concatenated gene
No. of haplotypes	20		32		24		21
Haplotype diversity (Hd)	0.832		0.998		0.979		0.966
Fu’s Fs statistic	−0.09404		−1.39769		−0.6347		−0.40481
Tajima’s D	−0.76952		−1.05145		−0.11697		−0.29339
K _a	0.07327		0.09485		0.17167		0.12331
K _s	0.56524		0.56374		0.75719		0.68005
K_a/K_s ratio	0.13		0.168		0.227		0.181
A (%)	33.03		31.23		46.12		31.93
T/U (%)	40.58		42.66		31.12		43.59
G (%)	10.29		14.01		13.62		9.75
C (%)	16.1		12.1		9.14		14.74

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