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Sensitivity of T. gondii detection by RAA-Cas13a-LFD and qPCR assay. (A) Visual inspection of RAA-Cas13a-LFD. (B) Curves for qPCR. Strip and curves: 1–10: plasmid copy number diluted to 1 ng/μL–1 × 10−9 ng/μL, respectively; NTC: negative control (ddH2O).
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