Fluorescence amplification curve of primer and probe combination. (A) The fluorescence amplification curve of Primer 1 + Probe 1 is very high in the positive plasmid control of T. gondii for three repeats; (B) The fluorescence curve of Primer 1 + Probe 3–4-a and Primer 3 + Probe 3–4-a indicates no significant amplification, and the consistency is poor for three repeats; (C) The fluorescence curve of Primer 1 + Probe 3–4-b and Primer 3 + Probe 3–4-b also shows no significant amplification, and the amplification consistency of Primer 1 + Probe 3–4-b is poor for the three repeats. PP: positive plasmid control; NTC: negative control.