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Schematic for T. gondii detection with the RAA-Cas13a-LFD assay. The procedures include amplification of target DNA with RAA; T7 RNA polymerase transcription of amplified DNA to RNA; target recognition of the target RNA with Cas13a-crRNA, collateral cleavage of fluorophore-quencher reporter (i.e., lateral flow reporter) and release fluorophore signal; visualization of the generated fluorescence signal by lateral flow dipstick.
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