Anatomical distribution of Toxoplasma gondii in naturally and experimentally infected lambs

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Table 3

Detectable parasite burden (arithmetic mean of T. gondii parasites per gram of tissue and range) from experimentally (E) infected lambs in fresh (Fre) and frozen (Fro) digest. NR: negative results (CT value > 40).

Tissue	E1	Fre	Fro	E2	Fre	Fro	E3	Fre	Fro	Fre	Fro
	g^a	p/g^b		g^a	p/g^b		g^a	p/g^b		Arithmetic mean	Arithmetic mean
										Range [min–max]	Range [min–max]
Lung	125.00	57.49	18.32	100.00	3.47	0.08	108.85	0.07	0.86	20.34	6.42
										[0.07–57.49]	[0.08–18.32]
Intestine	25.69	156.59	1.10	48.20	74.38	5.16	111.25	1.20	0.22	77.39	2.16
										[1.20–156.59]	[0.22–5.16]
Heart	65.15	2952.82	1307.39	60.65	724.44	181.02	60.40	195.03	49.00	1290.76	512.47
										[195.03–2952.82]	[49.00–1307.39]
Tongue	38.23	3700.57	63.80	40.80	872.63	159.30	62.00	573.86	72.92	1715.69	98.67
										[573.86–3700.57]	[63.80–159.31]
Deltoid	14.67	2715.38	78.19	49.61	2172.44	509.70	87.70	1737.06	671.91	2208.29	419.93
										[1737.06–2715.38]	[78.19–671.91]
Intercostal muscles	96.00	6845.06	202.37	141.45	692.60	349.91	179.05	423.13	258.13	2653.60	270.14
Intercostal muscles										[423.13–6845.06]	[202.37–349.91]
Diaphragm	43.80	6145.29	162.73	63.05	1346.90	91.98	53.10	763.98	123.44	2752.06	126.05
										[763.98–6145.29]	[91.98–162.73]
Quadriceps femoris	120.00	11,817.25	4185.37	133.50	1036.48	1334.73	140.60	991.93	599.01	4615.22	2039.70
Quadriceps femoris										[991.93–11,817.25]	[599.01–4185.37]
Brain	53.79	26,754.09	1649.42	54.50	2243.78	86.94	55.25	18.79	46.41	9672.22	594.2
										[18.79–26,754.09]	[46.41–1649.42]

g: grams of tissue.

p/g: calculated number of parasites per gram.

The number of T. gondii parasites was calculated by interpolating the average Ct values on the standard curve equivalent to 1 × 10⁰ to 1 × 10³ tachyzoites with tenfold serial dilutions. Estimated parasite numbers in tissue samples (detectable parasite burden) were expressed as parasite number/g lamb tissue. The range describes the lowest and highest estimated number of parasites within samples.

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