Table 2
Detectable parasite burden (arithmetic mean of T. gondii parasites per gram of tissue and range) from naturally (N) infected lambs in fresh (Fre) and frozen (Fro) digest. NR: negative results (CT value > 40).
| Tissue | N1 |
Fre |
Fro |
N2 |
Fre |
Fro |
N3 |
Fre |
Fro |
Fre |
Fro |
|---|---|---|---|---|---|---|---|---|---|---|---|
| ga | p/gb | ga | p/gb | ga | p/gb | Arithmetic mean Range [min–max] | Arithmetic mean Range [min–max] | ||||
| Intestine | 52.00 | NR | NR | 59.50 | 0.05 | NR | 130.00 | 0.02 | 0.10 | 0.02 [0.00–0.05] | 0.03 [0.00–0.10] |
| Tongue | 29.00 | NR | NR | 35.00 | NR | 0.17 | 49.50 | 0.15 | NR | 0.05 [0.00–0.15] | 0.06 [0.00–0.17] |
| Heart | 34.50 | 0.47 | NR | 41.10 | 0.19 | 0.26 | 49.33 | 0.14 | 0.19 | 0.30 [0.14–0.47] | 0.15 [0.00–0.26] |
| Quadriceps femoris | 140.00 | 0.24 | 0.15 | 140.00 | 0.11 | 1.12 | 175.00 | 0.64 | 0.04 | 0.33 [0.11–0.64] | 0.44 [0.04–1.12] |
| Lung | 52.00 | NR | 0.16 | 93.77 | 1.11 | NR | 86.50 | 0.04 | 0.24 | 0.38 [0.00–1.11] | 0.13 [0.00–0.24] |
| Intercostal muscles | 82.50 | 1.13 | 0.39 | 93.50 | 0.29 | 0.54 | 125.00 | 0.07 | 0.26 | 0.50 [0.07–1.13] | 0.40 [0.26–0.54] |
| Deltoid | 104.00 | 0.10 | 0.19 | 125.00 | 0.04 | 0.37 | 125.00 | 1.92 | 0.56 | 0.69 [0.04–1.92] | 0.37 [0.19–0.56] |
| Brain | 54.25 | NR | NR | 51.00 | 2.30 | 1.73 | 51.90 | NR | NR | 0.77 [0.00–2.30] | 0.58 [0.00–1.73] |
| Diaphragm | 19.50 | NR | NR | 57.26 | 0.04 | NR | 48.25 | 6.66 | 0.10 | 2.23 [0.00–6.66] | 0.03 [0.00–0.10] |
The number of T. gondii parasites was calculated by interpolating the average Ct values on the standard curve equivalent to 1 × 100 to 1 × 103 tachyzoites with tenfold serial dilutions. Estimated parasite numbers in tissue samples (detectable parasite burden) were expressed as parasite number/g lamb tissue. The range describes the lowest and highest estimated number of parasites within samples.
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