Splenic parasitism evaluated by RT-PCR. Splenic parasitism was also evaluated by RT-PCR, one and 15 days after treatment. Results were converted into number of parasites (in log) per nucleated cell (multiplied by one thousand to facilitate visualization). Bars indicate the mean ± standard deviation of the groups. (*), (^#) and (^Δ) indicate statistically significant differences in comparison to the saline, Mic/B and miltefosine groups, respectively (p < 0.05).