Humoral response developed in the treated and infected animals. Serum samples were collected from the treated and infected animals, one (A) and 15 (B) days after treatment, when the anti-parasite IgG1 and IgG2a isotype levels were evaluated. White and grey bars indicate the mean ± standard deviation of the IgG1 and IgG2a levels, respectively. (*) indicates a statistically significant difference in relation to the saline and Mic/B groups (p < 0.001). (⁺) indicates a statistically significant difference in relation to the miltefosine, ICHQ, and ICHQ/Mic groups (p < 0.001).