Cellular profile generated in the treated and infected animals, 15 days after treatment. Fifteen days after treatment, cellular response was also evaluated in the treated and infected animals. Spleen cells (5 × 10⁶ per mL) were non-stimulated (medium) or stimulated with Leishmania infantum SLA (50 μg/mL) for 48 h at 37 °C in 5% CO₂. IFN-γ, IL-4, IL-10, IL-12p70 and GM-CSF levels were measured in the cell supernatant by a capture ELISA (A). Nitrite production was also evaluated in the cell supernatant (B). The ratios between the IFN-γ and IL-10 levels were calculated, and values are also shown (C). Bars represent the mean ± standard deviation of the groups. (*) indicates a statistically significant difference in relation to the saline and Mic/B groups (p < 0.001). (⁺) indicates a statistically significant difference in relation to the miltefosine, ICHQ, and ICHQ/Mic groups (p < 0.001).